Structure-switchable aptamer-arranged reconfigurable DNA nanonetworks for targeted cancer therapy

The structural DNA nanotechnology holds great potential application in bioimaging, drug delivery and cancer therapy. Herein, an intelligent aptamer-incorporated DNA nanonetwork (Apt-Nnes) is demonstrated for cancer cell imaging and targeted drug delivery, which essentially is a micron-scale pattern with the thickness of double-stranded monolayer. Cancer cell-surface receptors can make it perform magical transformation into small size of nanosheet intermediates and specifically enter target cells. The binding affinity of Apt-Nnes is increased by 3-fold due to multivalent binding effect of aptamers and it can maintain the structural integrity in fetal bovine serum (FBS) for 8 h. More interestingly, target cancer cells can cause the structural disassembly, and each resulting unit transports 4963 doxorubicin (Dox) into target cells, causing the specific cellular cytotoxicity. The cell surface receptor-mediated disassembly of large size of DNA nanostructures into small size of fractions provides a valuable insight into developing intelligent DNA nanostructure suitable for biomedical applications.(c) 2022 Elsevier Inc. All rights reserved.

Automated summary

Structural DNA nanotechnology has great potential in applications such as bioimaging, drug delivery, and cancer therapy. This study demonstrates an intelligent aptamer-incorporated DNA nanonetwork for cancer cell imaging and targeted drug delivery. The nanonetwork can transform into nanosheet intermediates and specifically enter target cells through cancer cell-surface receptors, showing increased binding affinity and maintaining structural integrity.