4.8 Article

Electrically controlled mRNA delivery using a polypyrrole-graphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells

期刊

NANO RESEARCH
卷 15, 期 10, 页码 9253-9263

出版社

TSINGHUA UNIV PRESS
DOI: 10.1007/s12274-022-4613-y

关键词

messenger ribonucleic acid (mRNA) delivery; polypyrrole; graphene oxide; electrical release; mesenchymal stem cells

资金

  1. National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2019M3A9H2031820, NRF-2021R1A2C1010747, NRF-2022R1A2C4002217]
  2. Korean Fund for Regenerative Medicine - Ministry of Science and ICT
  3. Ministry of Health and Welfare [RS-2022-00070316]
  4. Bio & Medical Technology Development Program - Ministry of Science, ICT and Future Planning, Republic of Korea [NRF-2017M3A9E4047243]

向作者/读者索取更多资源

Electrically controlled mRNA delivery via a conductive hybrid film can guide osteogenic differentiation of human adipose-derived mesenchymal stem cells. This platform, capable of loading various types of RNAs, shows promise as a new nucleic acid delivery tool for stem cell-based therapeutics.
Direct messenger ribonucleic acid (mRNA) delivery to target cells or tissues has revolutionized the field of biotechnology. However, the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded nanocarriers. Herein, we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) via electrically controlled mRNA delivery. To find optimal electrical conductivity and mRNA-loading capacity, the polypyrrole-graphene oxide (PPy-GO) hybrid film was electropolymerized on indium tin oxide substrates. We found that the fluorescein sodium salt, a molecule partially mimicking the physical and chemical properties of mRNAs, can be effectively absorbed and released by electrical stimulation (ES). The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical stimulation. This platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.

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