期刊
NANO LETTERS
卷 22, 期 15, 页码 6391-6401出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.2c02259
关键词
microneedle; three-dimensional culture; mesenchymal stem cell; exosomes; spinal cord injury
类别
资金
- China Postdoctoral Science Foundation [2021M691977]
- Jinan Clinical Science and Technology Innovation Program [202019137, 202019138]
- National Natural Science Foundation of China [81972340, 82173140]
- Key project of Shandong Provincial Natural Science Foundation [ZR202010300086]
- Academic promotion program of Shandong First Medical University [2019LJ005]
Exosomes derived from three-dimensional culture of MSCs have shown great potential in spinal cord injury therapy, compared to exosomes from conventional two-dimensional culture. It is urgent to develop a reliable, convenient, and effective exosome delivery method for constant in situ release.
Exosomes derived from mesenchymal stem cells (MSCs) have been proven to exhibit great potentials in spinal cord injury (SCI) therapy. However, conventional two-dimensional (2D) culture will inevitably lead to the loss of stemness of MSCs, which substantially limits the therapeutic potency of MSCs exosomes (2D-Exo). Exosomes derived from three-dimensional culture (3D-Exo) possess higher therapeutic efficiency which have wide applications in spinal cord therapy. Typically, conventional exosome therapy that relies on local repeated injection results in secondary injury and low efficiency. It is urgent to develop a more reliable, convenient, and effective exosome delivery method to achieve constant in situ exosomes release. Herein, we proposed a controlled 3D-exohydrogel hybrid microneedle array patch to achieve SCI repair in situ. Our studies suggested that MSCs with 3D-culturing could maintain their stemness, and consequently, 3D-Exo effectively reduced SCI-induced inflammation and glial scarring. Thus, it is a promising therapeutic strategy for the treatment of SCI.
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