期刊
MOLECULES
卷 27, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/molecules27134282
关键词
ent-abietane diterpenoids; Euphorbia fischeriana; anti-breast cancer activity; response surface methodology; salting-out-assisted liquid-liquid extraction
资金
- Qiqihar Medical University [2021-ZDPY012, QMSI2019M-10]
- University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province [UNPYSCT-2018031, UNPYSCT-2018030]
- Qiqihar Science and Technology Bureau [LHYD-202002, LHYD-202003]
- Natural Science Foundation of China [81903865]
- Education Department of Heilongjiang Province [2017-KYYWF-0702]
Ent-abietane diterpenoids from Euphorbia fischeriana exhibit varying degrees of growth inhibition against breast cancer cell lines, with some compounds being novel or first-time discoveries. Extraction is crucial for obtaining valuable compounds from natural plants, and optimizing the salting-out-assisted liquid-liquid extraction process can maximize yields.
Ent-abietane diterpenoids are the main active constituents of Euphorbia fischeriana. In the continuing search for new anti-breast cancer drugs, 11 ent-abietane diterpenoids (1-11) were isolated from E. fischeriana. The structures of these compounds were clearly elucidated on the basis of 1D and 2D NMR spectra as well as HRESIMS data. Among them, compound 1 was a novel compound, compound 10 was isolated from Euphorbia genus for the first time, compound 11 was firstly discovered from E. fischeriana. These compounds exhibited varying degrees of growth inhibition against the MCF-10A, MCF-7, ZR-75-1 and MDA-MB-231 cell lines in vitro. The experimental data obtained permit us to identify the roles of the epoxy group, hydroxyl group and acetoxyl group on their cytotoxic activities. Extraction is an important means for the isolation, identification, and application of valuable compounds from natural plants. To maximize yields of ent-abietane diterpenoids of E. fischeriana, 17-hydroxyjolkinolide B, jolkinolide B, 17-hydroxyjolkinolide A and jolkinolide A were selected as quality controls to optimize the salting-out-assisted liquid-liquid extraction (SALLE) by response surface methodology (RSM). The optimized conditions for SALLE were 0.47 g sodium dihydrogen phosphate, 5.5 mL acetonitrile and 4.5 mL water at pH 7.5. The experimental values of 17-hydroxyjolkinolide B, jolkinolide B, 17-hydroxyjolkinolide A and jolkinolide A (2.134, 0.529, 0.396, and 0.148 mg/g, respectively) were in agreement with the predicted values, thus demonstrating the appropriateness of the model.
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