期刊
MOLECULES
卷 27, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/molecules27134042
关键词
chemical ecology; GCxGC; Gonipterus platensis; MD-GC; pheromones
资金
- National Funds through FCT-Foundation for Science and Technology [PDQS/BD/2022]
- Associate Laboratory for Green Chemistry-LAQV - FCT/MCTES [UID/QUI/50006/2020, UIDB/05183/2020]
- CENSE (Center for Environmental and Sustainability Research) - FCT/MCTES [UID/AMB/04085/2020]
- PDR2020 project GO-fitoglobulus [PDR2020-1.0.1-FEADER-031159]
- CEF - Foundation for Science and Technology (FCT), Portugal [UID/AGR/00239/2019, UIDB/00239/2020]
- HOMED project (H2020) [771271]
Multidimensional gas chromatography is a powerful analytical tool that allows for precise identification of compounds in complex matrices. The research on host selection behavior of the eucalyptus weevil discovered an attractive pheromone blend for both male and female weevils. By combining different techniques, relevant compounds were successfully selected and identified.
Multidimensional gas chromatography is, presently, an established and powerful analytical tool, due to higher resolving power than the classical 1D chromatographic approaches. Applied to multiple areas, it allows to isolate, detect and identify a larger number of compounds present in complex matrices, even in trace amounts. Research was conducted to determine which compounds, emitted by host plants of the eucalyptus weevil, Gonipterus platensis, might mediate host selection behavior. The identification of a pheromone blend of G. platensis is presented, revealing to be more attractive to weevils of both sexes, than the individual compounds. The volatile organic compounds (VOCs) were collected by headspace solid phase microextraction (HS-SPME), MonoTrap (TM) disks, and simultaneous distillation-extraction (SDE). Combining one dimensional (1D) and two-dimensional (2D) chromatographic systems-comprehensive and heart-cut two-dimensional gas chromatography (GCxGC and H/C-MD-GC, respectively) with mass spectrometry (MS) and electroantennographic (EAD) detection, enabled the selection and identification of pertinent semiochemicals which were detected by the insect antennal olfactory system. The behavioral effect of a selected blend of compounds was assessed in a two-arm olfactometer with ten parallel walking chambers, coupled to video tracking and data analysis software. An active blend, composed by cis and trans-verbenol, verbenene, myrtenol and trans-pinocarveol was achieved.
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