Antiviral Activity of Porcine IFN-λ3 and IFN-α against Porcine Rotavirus In Vitro

Interferons (IFNs) play a major role in the host's antiviral innate immunity. In response to viral infection, IFNs bind their receptors and initiate a signaling cascade, leading to the accurate transcriptional regulation of hundreds of IFN-stimulated genes (ISGs). Porcine rotavirus (PoRV) belongs to genus Rotavirus of the Reoviridae family; the infection is a global epidemic disease and a major threat to the pig industry. In this study, we found that IFN-lambda 3 inhibited the replication of PoRV in both MA104 cells and IPEC-J2 cells, and this inhibition was dose-dependent. Furthermore, the antiviral activity of IFN-lambda 3 was more potent in IPEC-J2 cells than in MA104 cells. Further research showed that IFN-lambda 3 and IFN-alpha might inhibit PoRV infection by activating ISGs, i.e., MxA, OASL and ISG15, in IPEC-J2 cells. However, the co-treatment of IFN-lambda 3 and IFN-alpha did not enhance the antiviral activity. Our data demonstrated that IFN-lambda 3 had antiviral activity against PoRV and may serve as a useful antiviral candidate against PoRV, as well as other viruses in swine.

Automated summary

IFN-lambda 3 exhibits antiviral activity against porcine rotavirus and may serve as a useful antiviral candidate.