期刊
MOLECULES
卷 27, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/molecules27144407
关键词
Guatteria olivacea; essential oil; liver cancer; antitumor
资金
- Brazilian agency Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [001]
- Brazilian agency Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [307619/2021-4]
- Brazilian agency CNPq/INCT [465357/2014]
- Brazilian agency Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM)
- Brazilian agency Fundacao Oswaldo Cruz (FIOCRUZ)
- Brazilian agency Universidade Federal do Amazonas (UFAM)
In this study, the cytotoxic and antitumor effects of the essential oil obtained from Guatteria olivacea leaves against liver cancer were evaluated. The essential oil exhibited potential in inhibiting liver cancer growth by inducing apoptosis and DNA fragmentation, without affecting reactive oxygen species levels.
Guatteria olivacea R. E. Fries (synonym Guatteria punctata (Aubl.) R.A. Howard) is a tree of 10-27 m tall popularly known as envira-bobo, envira-fofa, envireira, embira, embira-branca, embira-preta, envira-branca, and envira-preta, which can be found in the Brazilian Amazon biome. In this study, we evaluated the cytotoxic and antitumor effects of the essential oil (EO) obtained from the leaves of G. olivacea against liver cancer using HepG2 cells as a model. EO was obtained using a hydrodistillation Clevenger-type apparatus and was qualitatively and quantitatively characterized using GC-MS and GC-FID, respectively. The alamar blue assay was used to assess the cytotoxic potential of EO in a panel of human cancer cell lines and human non-cancerous cells. In HepG2 cells treated with EO, YO-PRO-1/propidium iodide staining, cell cycle distribution, and reactive oxygen species (ROS) were examined. In C.B-17 SCID mice with HepG2 cell xenografts, the efficacy of the EO (20 and 40 mg/kg) was tested in vivo. GC-MS and GC-FID analyses showed germacrene D (17.65%), 1-epi-cubenol (13.21%), caryophyllene oxide (12.03%), spathulenol (11.26%), (E)-caryophyllene (7.26%), bicyclogermacrene (5.87%), and delta-elemene (4.95%) as the major constituents of G. olivacea leaf EO. In vitro cytotoxicity of EO was observed, including anti-liver cancer action with an IC50 value of 30.82 mu g/mL for HepG2 cells. In HepG2 cells, EO treatment increased apoptotic cells and DNA fragmentation, without changes in ROS levels. Furthermore, the EO inhibited tumor mass in vivo by 32.8-57.9%. These findings suggest that G. olivacea leaf EO has anti-liver cancer potential.
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