Interdiction in the Early Folding of the p53 DNA-Binding Domain Leads to Its Amyloid-Like Misfolding

In this article, we investigate two issues: (a) the initial contact formation events along the folding pathway of the DNA-binding domain of the tumor suppressor protein p53 (core p53); and (b) the intermolecular events leading to its conversion into a prion-like form upon incubation with peptide P8(250-257). In the case of (a), the calculations employ the sequential collapse model (SCM) to identify the segments involved in the initial contact formation events that nucleate the folding pathway. The model predicts that there are several possible initial non-local contacts of comparative stability. The most stable of these possible initial contacts involve the protein segments (159)AMAIY(163) and (ILTII255,)-I-251 and it is the only native-like contact. Thus, it is predicted to constitute Nature's shortcut to the native structure of the core domain of p53. In the case of issue (b), these findings are then combined with experimental evidence showing that the incubation of the core domain of p53 with peptide P8(250-257), which is equivalent to the native protein segment (PILTIITL257)-P-250, leads to an amyloid conformational transition. It is explained how the SCM predicts that P8(250-257) effectively interdicts in the formation of the most stable possible initial contact and, thereby, disrupts the subsequent normal folding. Interdiction by polymeric P8(250-257) seeds is also studied. It is then hypothesized that enhanced folding through one or several of the less stable contacts could play a role in P8(250-257)-promoted core p53 amyloid misfolding. These findings are compared to previous results obtained for the prion protein. Experiments are proposed to test the hypothesis presented regarding core p53 amyloid misfolding.

Automated summary

This article investigates the initial contact formation events along the folding pathway of the DNA-binding domain of p53 and the intermolecular events leading to its conversion into a prion-like form upon incubation with peptide P8(250-257). The calculations employ the sequential collapse model (SCM) to identify the segments involved in the initial contacts formation. Experimental evidence shows that the incubation of p53 with peptide P8(250-257) leads to an amyloid conformational transition. The findings suggest that disrupted initial contacts and enhanced folding through less stable contacts may contribute to core p53 amyloid misfolding.