4.7 Article

NMR Reveals Functionally Relevant Thermally Induced Structural Changes within the Native Ensemble of G-CSF

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Medicine, Research & Experimental

Protein Engineering and HDX Identify Structural Regions of G-CSF Critical to Its Stability and Aggregation

Victoria E. Wood et al.

Summary: Protein engineering and formulation for therapeutic proteins present a major challenge in the biopharmaceutical industry due to the need for prolonged shelf-life. Understanding the impact of mutations and formulations on protein structure and dynamics can lead to more predictive approaches for improvement. Studies using intrinsic fluorescence analysis and hydrogen deuterium exchange mass spectrometry have revealed insights into the reorganization and stabilization of specific protein loops, providing valuable information for enhancing protein stability and reducing aggregation rates.

MOLECULAR PHARMACEUTICS (2022)

Article Biochemistry & Molecular Biology

Investigation of the pH-dependent aggregation mechanisms of GCSF using low resolution protein characterization techniques and advanced molecular dynamics simulations

Suk Kyu Ko et al.

Summary: This study used state-of-the-art simulation techniques to model the complex pH-dependent aggregation behavior of GCSF. The results showed that the conformational stability and protein-protein interactions of GCSF play important roles in aggregation, and colloidal stability is also a crucial factor affecting GCSF aggregation.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2022)

Article Biochemistry & Molecular Biology

Machine learning reveals hidden stability code in protein native fluorescence

Hongyu Zhang et al.

Summary: By training a neural network model to predict spectra based on the temperature-dependence of intrinsic fluorescence emission under native conditions, hidden information related to stability was discovered in native fluorescence spectra. This method could potentially enable rapid screening of therapeutic protein variants and formulations based on spectroscopic measurements at non-denaturing temperatures.

COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (2021)

Article Biochemistry & Molecular Biology

Evaluation of Non-Uniform Sampling 2D 1H-13C HSQC Spectra for Semi-Quantitative Metabolomics

Bo Zhang et al.

METABOLITES (2020)

Article Chemistry, Physical

Dynamics of the Proline-Rich C-Terminus of Huntingtin Exon-1 Fibrils

Bethany G. Caulkins et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2018)

Article Multidisciplinary Sciences

Ubiquitin S65 phosphorylation engenders a pH-sensitive conformational switch

Xu Dong et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)

Article Cell & Tissue Engineering

THE EFFECT OF EXTRACELLULAR ACIDOSIS ON THE BEHAVIOUR OF MESENCHYMAL STEM CELLS IN VITRO

A. Massa et al.

EUROPEAN CELLS & MATERIALS (2017)

Article Medicine, Research & Experimental

A comparison of biophysical characterization techniques in predicting monoclonal antibody stability

Geetha Thiagarajan et al.

Article Multidisciplinary Sciences

Direct measurement of local oxygen concentration in the bone marrow of live animals

Joel A. Spencer et al.

NATURE (2014)

Article Biochemistry & Molecular Biology

PASTA 2.0: an improved server for protein aggregation prediction

Ian Walsh et al.

NUCLEIC ACIDS RESEARCH (2014)

Article Multidisciplinary Sciences

A Consensus Method for the Prediction of 'Aggregation-Prone' Peptides in Globular Proteins

Antonios C. Tsolis et al.

PLOS ONE (2013)

Review Biochemistry & Molecular Biology

An introduction to NMR-based approaches for measuring protein dynamics

Ian R. Kleckner et al.

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS (2011)

Article Pharmacology & Pharmacy

Predicting solution aggregation rates for therapeutic proteins: Approaches and challenges

Christopher J. Roberts et al.

INTERNATIONAL JOURNAL OF PHARMACEUTICS (2011)

Article Biochemistry & Molecular Biology

Residual dipolar couplings as a tool to study molecular recognition of ubiquitin

Nils-Alexander Lakomek et al.

BIOCHEMICAL SOCIETY TRANSACTIONS (2008)

Review Chemistry, Multidisciplinary

Characterization of the fast dynamics of protein amino acid side chains using NMR relaxation in solution

Tatyana I. Igumenova et al.

CHEMICAL REVIEWS (2006)

Article Multidisciplinary Sciences

Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex

T Tamada et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Article Biochemistry & Molecular Biology

The CCPN data model for NMR spectroscopy: Development of a software pipeline

WF Vranken et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2005)

Article Biochemical Research Methods

Protein folding studied by real-time NMR spectroscopy

M Zeeb et al.

METHODS (2004)

Article Biochemistry & Molecular Biology

Potential bias in NMR relaxation data introduced by peak intensity analysis and curve fitting methods

JH Viles et al.

JOURNAL OF BIOMOLECULAR NMR (2001)

Review Biochemistry & Molecular Biology

Native disulfide bond formation in proteins

KJ Woycechowsky et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2000)