期刊
MOLECULAR ONCOLOGY
卷 16, 期 20, 页码 3642-3653出版社
WILEY
DOI: 10.1002/1878-0261.13300
关键词
aerobic glycolysis; autophagy; cancer; HIF-1; S1P-lyase; sphingosine-1-phosphate
类别
资金
- Deutsche Forschungsgemeinschaft (DFG) [EC-118/10-1]
- Projekt DEAL
The study revealed that SGPL1-deficient cells tend to convert glucose to lactate under the influence of S1P, leading to increased proliferation rate, but without negatively affecting cellular energy status. Activation of the Akt/mTOR pathway and down-regulation of autophagy were identified as key mechanisms underlying these changes.
Sphingosine-1-phosphate (S1P), a bioactive signaling lipid, is involved in several vital processes, including cellular proliferation, survival and migration, as well as neovascularization and inflammation. Its critical role in the development and progression of cancer is well documented. The metabolism of S1P, which exerts its effect mainly via five G protein-coupled receptors (S1PR(1-5)), is tightly regulated. S1P-lyase (SGPL1) irreversibly cleaves S1P in the final step of sphingolipid catabolism and exhibits remarkably decreased enzymatic activity in tumor samples. In this study, we used SGPL1-deficient (Sgpl1(-/-)) mouse embryonic fibroblasts (MEFs) and investigated the impact of S1P on glucose metabolism. Accumulated S1P activates, via its receptors (S1PR(1-3)), hypoxia-inducible factor 1 and stimulates the expression of proteins involved in glucose uptake and breakdown, indicating that Sgpl1(-/-) cells, like cancer cells, prefer to convert glucose to lactate even in the presence of oxygen. Accordingly, their rate of proliferation is significantly increased. Activation of the Akt/mTOR pathway and hence down-regulation of autophagy indicate that these changes do not negatively affect the cellular energy status. In summary, we report on a newly identified role of the S1P/S1PR(1-3) axis in glucose metabolism in SGPL1-deficient MEFs.
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