Plasma Matrix Metalloproeteinase-9 Is Associated with Seizure and Angioarchitecture Changes in Brain Arteriovenous Malformations

Both angiogenesis and inflammation contribute to activation of matrix metalloproeteinase-9 (MMP-9), which dissolves the extracellular matrix, disrupts the blood-brain barrier, and plays an important role in the pathogenesis of brain arteriovenous malformations (BAVMs). The key common cytokine in both angiogenesis and inflammation is interleukin 6 (IL-6). Previous studies have shown elevated systemic MMP-9 and decreased systemic vascular endothelial growth factor (VEGF) in BAVM patients. However, the clinical utility of plasma cytokines is unclear. The purpose of this study is to explore the relationship between plasma cytokines and the clinical presentations of BAVMs. Prospectively, we recruited naive BAVM patients without hemorrhage as the experimental group and unruptured intracranial aneurysm (UIA) patients as the control group. All patients received digital subtraction angiography, and plasma cytokines were collected from the lesional common carotid artery. Plasma cytokine levels were determined using a commercially available, monoclonal antibody-based enzyme-linked immunosorbent assay. Subgroup analysis based on hemorrhagic presentation and angiograchitecture was done for the BAVM group. Pearson correlations were calculated for the covariates. Means and differences for continuous and categorical variables were compared using Student's t and chi(2) tests respectively. Plasma MMP-9 levels were significantly higher in the BAVM group (42,945 +/- 29,991 pg/mL) than in the UIA group (28,270 +/- 17,119 pg/mL) (p < 0.001). Plasma MMP-9 levels in epileptic BAVMs (57,065 +/- 35,732; n = 9) were higher than in non-epileptic BAVMs (35,032 +/- 28,301; n = 19) (p = 0.049). Lower plasma MMP-9 levels were found in cases of BAVM with angiogenesis and with peudophlebitis. Plasma MMP-9 is a good biomarker reflecting ongoing vascular remodeling in BAVMs. Angiogenesis and pseudophlebitis are two angioarchitectural signs that reflect MMP-9 activities and can potentially serve as imaging biomarkers for epileptic BAVMs.

Automated summary

Both angiogenesis and inflammation contribute to the activation of matrix metalloproteinase-9 (MMP-9) in brain arteriovenous malformations (BAVMs), which plays a crucial role in the pathogenesis of BAVMs by dissolving the extracellular matrix and disrupting the blood-brain barrier. Interleukin 6 (IL-6) is identified as the key common cytokine in both angiogenesis and inflammation. This study aims to explore the relationship between plasma cytokines and the clinical presentations of BAVMs. The results indicate that plasma MMP-9 levels are significantly higher in BAVM patients compared to patients with unruptured intracranial aneurysms (UIAs), and are associated with epileptic BAVMs. Angiogenesis and pseudophlebitis can potentially serve as imaging biomarkers for epileptic BAVMs.