期刊
MOLECULAR ECOLOGY
卷 31, 期 18, 页码 4707-4725出版社
WILEY
DOI: 10.1111/mec.16611
关键词
climate change; differential gene expression; environmental variability; embiotocidae; global change; RNA-seq
资金
- David and Lucile Packard Foundation
- Packard Endowment Award via UCSC
- UCSC Committee on Research
This study reveals the neurological impacts of acidification on a temperate fish species and suggests that natural environmental variability may mitigate the effects of ocean acidification.
Acidification-induced changes in neurological function have been documented in several tropical marine fishes. Here, we investigate whether similar patterns of neurological impacts are observed in a temperate Pacific fish that naturally experiences regular and often large shifts in environmental pH/pCO(2). In two laboratory experiments, we tested the effect of acidification, as well as pH/pCO(2) variability, on gene expression in the brain tissue of a common temperate kelp forest/estuarine fish, Embiotoca jacksoni. Experiment 1 employed static pH treatments (target pH = 7.85/7.30), while Experiment 2 incorporated two variable treatments that oscillated around corresponding static treatments with the same mean (target pH = 7.85/7.70) in an eight-day cycle (amplitude +/- 0.15). We found that patterns of global gene expression differed across pH level treatments. Additionally, we identified differential expression of specific genes and enrichment of specific gene sets (GSEA) in comparisons of static pH treatments and in comparisons of static and variable pH treatments of the same mean pH. Importantly, we found that pH/pCO(2) variability decreased the number of differentially expressed genes detected between high and low pH treatments, and that interindividual variability in gene expression was greater in variable treatments than static treatments. These results provide important confirmation of neurological impacts of acidification in a temperate fish species and, critically, that natural environmental variability may mediate the impacts of ocean acidification.
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