4.5 Article

A microfluidic system for rapid nucleic acid analysis based on real-time convective PCR at point-of-care testing

期刊

MICROFLUIDICS AND NANOFLUIDICS
卷 26, 期 9, 页码 -

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s10404-022-02577-5

关键词

Microfluidic chip; Nucleic acid analysis; Mixing; Nucleic acid extraction; Convective PCR; Point-of-care (POC) testing

资金

  1. National Natural Science Foundation of China [81871505, 61971026]
  2. Fundamental Research Fund for the Central Universities [XK1802-4]
  3. National Science and Technology Major Project [2018ZX10732101-001-009]
  4. Beijing University of Chemical Technology [buctylkjcx06]

向作者/读者索取更多资源

A microfluidic system based on real-time convective PCR has been developed for rapid and automated nucleic acid extraction, amplification, and in-situ detection, demonstrating improved detection efficiency and accuracy.
A microfluidic system for rapid nucleic acid analysis based on real-time convective PCR is developed. To perform 'sample-in, answer-out' nucleic acid analysis, a microfluidic chip is developed to efficiently extract nucleic acid, and meanwhile convective PCR (CPCR) is applied for rapid nucleic acid amplification. With an integrated microfluidic chip consisting of reagent pre-storage chambers, a lysis & wash chamber, an elution chamber and a waste chamber, nucleic acid extraction based on magnetic beads can be automatically performed for a large size of test sample within a limited time. Based on an easy-to-operate strategy, different pre-stored reagents can be conveniently released for consecutive reaction at different steps. To achieve efficient mixing, a portable companion device is developed to introduce properly controlled 3-D actuation to magnetic beads in nucleic acid extraction. In CPCR amplification, PCR reagent can be spontaneously and repeatedly circulated between hot and cool zones of the reactor for space-domain thermal cycling based on pseudo-isothermal heating. A handheld real-time CPCR device is developed to perform nucleic acid amplification and in-situ detection. To extend the detection throughput, multiple handheld real-time CPCR devices can be grouped together by a common control system. It is demonstrated that influenza A (H1N1) viruses with the reasonable concentration down to 1.0 TCID50/ml can be successfully detected with the microfluidic system.

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