期刊
MICROCHIMICA ACTA
卷 189, 期 7, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-022-05338-x
关键词
Metal-organic framework; Laser desorption ionization mass spectrometry; Biomolecule determination; Glucose monitoring; Clinical diagnosis
资金
- National Natural Science Foundation of China [22004004, 21804123]
- Beijing Key Laboratory of Mental Disorders [2021JSJB02]
In this study, functional metal-organic frameworks (MOFs) were used as matrices in laser desorption ionization mass spectrometry (LDI-MS) for rapid detection of small biomolecules. The developed LDI-MS platform showed advantages of low sample consumption, short analysis time, strong salt tolerance, and satisfactory reproducibility, with potential applications in clinical diagnosis and disease monitoring.
A series of functional metal-organic frameworks (MOFs) were facilely prepared through an one-pot procedure or post-synthetic modification strategy and used as matrices in laser desorption ionization mass spectrometry (LDI-MS). Compared with traditional organic matrices and other MOFs, maltose-functional MOF MIL-101-maltose demonstrated ultrahigh ionization efficiency, free matrix background, uniform crystallization, and good dispersibility. A simple, general, and efficient LDI-MS platform was developed for rapid detection of various small biomolecules using MIL-101-maltose as matrix, providing several advantages including low sample consumption of 500 nL, short analysis time of few seconds, strong salt tolerance (500 mM NaCl), and satisfactory reproducibility. The MIL-101-maltose matrix was used for serum glucose determination and successfully distinguished the diabetic patients from the healthy controls. This work provides a generic LDI-MS platform for fast determination of small biomolecules with high potential in clinical diagnosis and disease monitoring.
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