Label-free electrochemical microfluidic biosensors: futuristic point-of-care analytical devices for monitoring diseases

The integration of microfluidics with electrochemical analysis has resulted in the development of single miniaturized detection systems, which allows the precise control of sample volume with multi-analyte detection capability in a cost- and time-effective manner. Microfluidic electrochemical sensing devices (MESDs) can potentially serve as precise sensing and monitoring systems for the detection of molecular markers in various detrimental diseases. MESDs offer several advantages, including (i) automated sample preparation and detection, (ii) low sample and reagent requirement, (iii) detection of multianalyte in a single run, (iv) multiplex analysis in a single integrated device, and (v) portability with simplicity in application and disposability. Label-free MESDs can serve an affordable real-time detection with a simple analysis in a short processing time, providing point-of-care diagnosis/detection possibilities in precision medicine, and environmental analysis. In the current review, we elaborate on label-free microfluidic biosensors, provide comprehensive insights into electrochemical detection techniques, and discuss the principles of label-free microfluidic-based sensing approaches.

Automated summary

The integration of microfluidics with electrochemical analysis has led to the development of single miniaturized detection systems with precise control of sample volume and multi-analyte detection capability. Microfluidic electrochemical sensing devices (MESDs) offer several advantages, including automated sample preparation and detection, low sample requirement, and the ability to detect multiple analytes. Label-free MESDs allow for affordable real-time detection and can be used for point-of-care diagnosis and environmental analysis.