期刊
MEDICAL AND VETERINARY ENTOMOLOGY
卷 36, 期 3, 页码 309-319出版社
WILEY
DOI: 10.1111/mve.12602
关键词
Aedes aegypti; arbovirus; Burkina Faso; dengue; insecticide resistance; kdr mutations; vector control
资金
- Medical Research Council of the UK/Global Challenges Research Fund [MR/P027873/1]
The response to recent dengue outbreaks in Burkina Faso relied on insecticide-based measures, but the susceptibility of the vector population to the insecticides used was not well understood. This study found that Aedes aegypti populations in Burkina Faso exhibited resistance to multiple insecticide classes, indicating the importance of monitoring insecticide resistance in national dengue control programs.
The response to recent dengue outbreaks in Burkina Faso was insecticide-based, despite poor knowledge of the vector population's susceptibility to the insecticides used. Here, we report on the susceptibility to the main insecticide classes and identify important underlying mechanisms in Aedes aegypti populations in Ouagadougou and Banfora, in 2019 and 2020. Wild Ae. aegypti were tested as adults in WHO bioassays and then screened in real time melting curve qPCR analyses to genotype the F1534C, V1016I, and V410L Aedes kdr mutations. Ae. aegypti showed moderate resistance to 0.1% bendiocarb (80-95% survival post-exposure), 0.8% Malathion (60-100%), 0.21% pirimiphos-methyl (75% - 97%), and high resistance to 0.03% deltamethrin (20-70%). PBO pre-exposure partially restored pyrethroid susceptibility. Genotyping detected high frequency of 1534C allele (0.92) and moderate 1016I (0.1-0.32). The V410L mutation was detected in Burkina Faso for the first time (frequency 0.1-0.36). Mosquitoes surviving 4 h exposure to 0.03% deltamethrin had significantly higher frequencies of the F1534C mutation than dead mosquitoes (0.70 vs. 0.96, p < 0.0001) and mosquitoes surviving 2 - 4 h exposure had a significantly reduced life span. Ae. aegypti from Burkina Faso are resistant to multiple insecticide classes with multiple mechanisms involved, demonstrating the essential role of insecticide resistance monitoring within national dengue control programmes.
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