期刊
MATERIALS & DESIGN
卷 220, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.matdes.2022.110829
关键词
Correlative imaging; Human carious enamel; Light microscopy; FIB-S(T)EM; Image analysis 2D; 3D; Synchrotron X-ray tomography
资金
- Engineering and Physical Sciences Research Council (EPSRC) [EP/P005381/1, EP/V007785/1]
- Medical Science Divisions, University of Oxford [BRR00060-DF02, BRR00060-DF03]
- Materials & Structural Analysis team from Thermo Fisher Scientific
This study investigates the nanoscale structural changes within dental carious lesions and establishes dental 3D nano-histology as an advanced platform for quantitatively evaluating caries-induced structural modification.
Dental caries is a widespread disease that proceeds by damaging superficial tooth enamel by heterogeneous dissolution. Conventional histology identifies different zones within carious lesions by their optical appearance, but fails to quantify the underlying nanoscale structural changes as a function of specific location, impeding better understanding of the demineralisation process. We employ detailed collocative analysis using different imaging modalities, resolutions and fields of view. Focused ion beam-scanning electron microscopy (FIB-SEM) reveals subsurface 3D nanostructure within milled micro-sized volumes, whilst X-ray tomography allows minimally destructive 3D imaging over large volumes. Correlative combination of these techniques reveals fine detail of enamel rods, inter-rod substance, sheaths, crystallites and voids as a function of location. The degree of enamel demineralisation within the body of the lesion, near its front, and at the surface is visualized and quantified in 3D. We thus establish the paradigm of dental 3D nano-histology as an advanced platform for quantitative evaluation of caries-induced structural modification.CO 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).
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