期刊
MACROMOLECULAR BIOSCIENCE
卷 22, 期 9, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.202200134
关键词
bacterial fimbriae; bioorthogonal; capsular antigen fragment 1; crosslinked hydrogel; protein polymer
资金
- Industrial Biotechnology Catalyst [BB/M018318/1]
- Junta de Comunidades de Castilla la Mancha
- EU [SB-PLY/19/180501/000191]
3D cell culture is superior to 2D in mimicking in vivo environments, and the use of Caf1 hydrogels as a cell culture platform shows promise in avoiding cytotoxicity and enabling successful 3D cell culture.
There is a growing realization that 3D cell culture better mimics complex in vivo environments than 2D, lessening aberrant cellular behaviors and ultimately improving the outcomes of experiments. Chemically crosslinked hydrogels which imitate natural extracellular matrix (ECM) are proven cell culture platforms, but the encapsulation of cells within these hydrogel networks requires bioorthogonal crosslinking chemistries which can be cytotoxic, synthetically demanding, and costly. Capsular antigen fragment 1 (Caf1) is a bacterial, polymeric, fimbrial protein which can be genetically engineered to imitate ECM. Furthermore, it can, reversibly, thermally interconvert between its polymeric and monomeric forms even when chemically crosslinked within a hydrogel network. It is demonstrated that this meltable feature of Caf1 hydrogels can be utilized to encapsulate neonatal human dermal fibroblasts at a range of cell densities (2 x 10(5)-2 x 10(6) cells mL(-1) of hydrogel) avoiding issues with chemical cytotoxicity. These hydrogels supported cell 3D culture for up to 21 d, successfully inducing cellular functions such as proliferation and migration. This work is significant because it further highlights the potential of simple, robust, Caf1-based hydrogels as a cell culture platform.
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