☆ 4.3 Article

Sensitive and high-accuracy detection of Salmonella based on CRISPR/Cas12a combined with recombinase polymerase amplification

LETTERS IN APPLIED MICROBIOLOGY (2022)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Review Chemistry, Analytical

Review of COVID-19 testing and diagnostic methods

Olena Filchakova et al.

Summary: More than six billion tests for COVID-19 have been conducted worldwide. This article emphasizes the importance of testing for the SARS-CoV-2 virus and corresponding human antibodies, not only for medical diagnosis and treatment, but also for economic and social activities. The review discusses the parameters of these tests and compares different detection methods. While RT-PCR remains the gold standard, there is a need for alternative methods that are less expensive and more accessible.

TALANTA (2022)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Evaluation of different target genes for the detection of Salmonella sp. by loop-mediated isothermal amplification

A. Kreitlow et al.

Summary: The LAMP technique was utilized to identify salmonella using six specific sequences as targets, with ttrRSBCA primers showing the best overall performance. Other primer sets had deficiencies, with some failing to detect certain Salmonella serotypes.

LETTERS IN APPLIED MICROBIOLOGY (2021)

添加到收藏夹

Article Biophysics

opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection

Rui Wang et al.

Summary: The opvCRISPR system integrates RT-LAMP and Cas12a cleavage to provide a rapid and sensitive detection method for SARS-CoV-2. Its ability to detect at nearly single molecule level in 45 minutes and show 100% agreement with traditional methods when validated with clinical samples make it a promising tool for point-of-care molecular diagnostics in the future.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Chemistry, Applied

RPA-Cas12a-FS: A frontline nucleic acid rapid detection system for food safety based on CRISPR-Cas12a combined with recombinase polymerase amplification

Hua Liu et al.

Summary: This study developed a detection technique by combining recombinase polymerase amplification with CRISPR-Cas12a for food safety, which can rapidly, simply, and highly sensitively detect foodborne pathogenic bacteria, genetically modified crops, and meat adulteration without requiring technical expertise or equipment.

FOOD CHEMISTRY (2021)

添加到收藏夹

Article Chemistry, Analytical

Ultrasensitive pathogenic bacteria detection by a smartphone-read G-quadruplex-based CRISPR-Cas12a bioassay

Lijuan Yin et al.

Summary: A G-quadruplex-based CRISPR-Cas12a bioassay was developed for high sensitivity and visualization detection of Salmonella. The method achieved a detection limit of 1 CFU/mL for Salmonella and successfully detected the bacteria in real food samples.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

添加到收藏夹

Article Virology

LAMP-Coupled CRISPR-Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses

Ahmed Mahas et al.

Summary: The article introduces a nucleic acid diagnostic method for detecting tomato yellow leaf curl virus and tomato leaf curl New Delhi virus using the CRISPR-Cas12a system. This method is rapid, sensitive, highly accurate, and suitable for on-site applications.

VIRUSES-BASEL (2021)

添加到收藏夹

Article Immunology

Development and Evaluation of the Rapid and Sensitive RPA Assays for Specific Detection of Salmonella spp. in Food Samples

Liwei Zhao et al.

Summary: The study utilized a simple, rapid, sensitive, and reliable real-time recombinase polymerase amplification technology to successfully detect Salmonella spp., with analysis time reduced to 5-12 minutes and equally reliable results compared to traditional PCR assays.

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (2021)

添加到收藏夹

Article Biophysics

An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for Listeria monocytogenes detection

Fan Li et al.

Summary: The study introduces the trans-cleavage activity of CRISPR/Cas12a into an electrochemical biosensor (ECRISPR) combined with recombinase-assisted amplification (RAA) to develop a cost-effective, specific, and ultrasensitive method for Listeria monocytogenes detection. The RAA-based E-CRISPR sensor can detect as low as 0.68 aM of genomic DNA and 26 cfu/mL of L. monocytogenes in pure cultures, demonstrating rapid and ultrasensitive detection capabilities in spiked and natural samples. This system shows no cross-reactivity with other non-target bacteria, making it a simple, highly sensitive, and accurate platform for L. monocytogenes detection.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Microbiology

Rapid and Sensitive Detection of Salmonella spp. Using CRISPR-Cas13a Combined With Recombinase Polymerase Amplification

Bailin An et al.

Summary: The study developed a simple, rapid, and specific detection method for Salmonella spp. using one-tube RPA-Cas13a, while the two-step assay was more sensitive, particularly for samples at low abundance.

FRONTIERS IN MICROBIOLOGY (2021)

添加到收藏夹

Article Chemistry, Applied

Ultrasensitive label-free immunochromatographic strip sensor for Salmonella determination based on salt-induced aggregated gold nanoparticles

Yarong Ren et al.

Summary: The innovative label-free ICTS sensor utilizes salt-induced aggregated gold nanoparticles as the signal probe for rapid identification of Salmonella, with the ability to show color difference even at low concentrations, providing excellent precision and recovery rates in real samples.

FOOD CHEMISTRY (2021)

添加到收藏夹

Article Food Science & Technology

Sensitive detection of foodborne pathogens based on CRISPR-Cas13a

Song Gao et al.

Summary: This study proposed a PCF detection method based on the CRISPR-Cas13a system for sensitive and specific detection of Salmonella, showing excellent sensitivity to detect Salmonella genomic DNA with a minimum of 10(1) aM or 10 degrees CFU/ml Salmonella bacteria within 2 hours. The method also demonstrated good specificity with no cross-reaction with other common foodborne bacteria.

JOURNAL OF FOOD SCIENCE (2021)

添加到收藏夹

Review Food Science & Technology

Biosensors for rapid detection of Salmonella in food: A review

Yafang Shen et al.

Summary: This review provides a comprehensive overview of Salmonella biosensors, highlighting different signal-transducing mechanisms and recent trends, especially in combination with advanced technologies. Furthermore, current challenges and future prospects are emphasized.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2021)

添加到收藏夹

Article Food Science & Technology

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Jiali Li et al.

FOODS (2020)

添加到收藏夹

Article Chemistry, Analytical

Integration of logic gates to CRISPR/Cas12a system for rapid and sensitive detection of pathogenic bacterial genes

Lei Peng et al.

ANALYTICA CHIMICA ACTA (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

A One-Pot Toolbox Based on Cas12a/crRNA Enables Rapid Foodborne Pathogen Detection at Attomolar Level

Yunqing Wang et al.

ACS SENSORS (2020)

添加到收藏夹

Article Microbiology

Validation of Droplet Digital Polymerase Chain Reaction forSalmonellaspp. Quantification

Carolina Villamil et al.

FRONTIERS IN MICROBIOLOGY (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

Enhancement of target specificity of CRISPR-Cas12a by using a chimeric DNA-RNA guide

Hanseop Kim et al.

NUCLEIC ACIDS RESEARCH (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection

Long T. Nguyen et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay

Xiong Ding et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

Rapid detection of SARS-CoV-2 with CRISPR-Cas12a

Dan Xiong et al.

PLOS BIOLOGY (2020)

添加到收藏夹

Article Chemistry, Analytical

A rapid and sensitive CRISPR/Cas12a based lateral flow biosensor for the detection of Epstein-Barr virus

Ting Yuan et al.

ANALYST (2020)

添加到收藏夹

Article Biochemical Research Methods

A lab-on-chip device for the sample-in-result-out detection of viable Salmonella using loop-mediated isothermal amplification and real-time turbidity monitoring

Siyuan Wang et al.

LAB ON A CHIP (2020)

添加到收藏夹

Review Immunology

Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods

Xinjie Wang et al.

EMERGING MICROBES & INFECTIONS (2020)

添加到收藏夹

Review Public, Environmental & Occupational Health

Infection, colonization and shedding of Campylobacter and Salmonella in animals and their contribution to human disease: A review

Elpidius Rukambile et al.

ZOONOSES AND PUBLIC HEALTH (2019)

添加到收藏夹

Article Biochemical Research Methods

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

Linxian Li et al.

ACS SYNTHETIC BIOLOGY (2019)

添加到收藏夹

Article Biotechnology & Applied Microbiology

CDetection: CRISPR-Cas12b-based DNA detection with sub-attomolar sensitivity and single-base specificity

Fei Teng et al.

GENOME BIOLOGY (2019)

添加到收藏夹

Article Biochemistry & Molecular Biology

Chimeric Guides Probe and Enhance Cas9 Biochemical Activity

Zachary J. Kartje et al.

BIOCHEMISTRY (2018)

添加到收藏夹

Review Food Science & Technology

A Meta-Analysis of Major Foodborne Pathogens in Chinese Food Commodities Between 2006 and 2016

Narayan Paudyal et al.

FOODBORNE PATHOGENS AND DISEASE (2018)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

添加到收藏夹

Letter Cell Biology

CRISPR-Cas12a-assisted nucleic acid detection

Shi-Yuan Li et al.

CELL DISCOVERY (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

Lucas B. Harrington et al.

SCIENCE (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Nucleic acid detection with CRISPR-Cas13a/C2c2

Jonathan S. Gootenberg et al.

SCIENCE (2017)

添加到收藏夹

Article Chemistry, Analytical

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Triggered Isothermal Amplification for Site-Specific Nucleic Acid Detection

Mengqi Huang et al.

ANALYTICAL CHEMISTRY (2017)

添加到收藏夹

Correction Biochemistry & Molecular Biology

Guidelines for diagnostic next-generation sequencing (vol 24, pg 2, 2016)

Gert Matthijs et al.

EUROPEAN JOURNAL OF HUMAN GENETICS (2016)

添加到收藏夹

Review Chemistry, Multidisciplinary

Isothermal Amplification of Nucleic Acids

Yongxi Zhao et al.

CHEMICAL REVIEWS (2015)

添加到收藏夹

Review Pathology

Nucleic acid detection technologies and marker molecules in bacterial diagnostics

Ott Scheler et al.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2014)

添加到收藏夹

Article Multidisciplinary Sciences

CRISPR provides acquired resistance against viruses in prokaryotes

Rodolphe Barrangou et al.

SCIENCE (2007)

添加到收藏夹

Article Immunology

Characterization of FimY as a coactivator of type 1 fimbrial expression in Salmonella enterica serovar typhimurium

JK Tinker et al.

INFECTION AND IMMUNITY (2000)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.