期刊
KOREAN JOURNAL OF PARASITOLOGY
卷 60, 期 3, 页码 201-206出版社
KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI
DOI: 10.3347/kjp.2022.60.3.201
关键词
Babesia microti; recombinase-aided amplification; molecular detection
类别
资金
- Department of Science and Technology of Jiangsu Province [BE2019755, BZ2020003]
In this study, a rapid, sensitive, and specific detection method for B. microti was developed using recombinase-aided amplification (RAA) assay, which showed promising results and potential application in screening potentially infected individuals.
Babesia microti is one of the most common causative agents of babesiosis. A sensitive and rapid detection is necessary for screening potentially infected individuals. In this study, B. microti cytochrome c oxidase subunit I (cox1) was selected as the target gene, multiple primers were designed, and optimized by a recombinase-aided amplification (RAA) assay. The optimal primers and probe were labeled with fluorescein. The sensitivity of fluorescent RAA (fRAA) was evaluated using gradient diluents of the cox1 recombinant plasmid and genomic DNA extracted from whole blood of B. microti infected mice. The specificity of fRAA was assessed by other transfusion transmitted parasites. The analytical sensitivity of the fRAA assay was 10 copies of recombinant plasmid per reaction and 10 fg/??l B. microti genomic DNA. No cross-reaction with any other blood-transmitted parasites was observed. Our results demonstrated that the fRAA assay would be rapid, sensitive, and specific for the detection of B. microti.
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