期刊
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
卷 34, 期 4, 页码 733-737出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/10406387221102123
关键词
BoLA; bovine leukemia virus; cattle; enzootic bovine leukosis; Japan; methylation
资金
- JSPS KAKENHI [20H03142, 20J10567]
- Grants-in-Aid for Scientific Research [20H03142, 20J10567] Funding Source: KAKEN
This study investigated a case of enzootic bovine leukosis in a Japanese Black beef cow, identifying various pathological and genetic features associated with the disease.
A 21-mo-old Japanese Black beef cow had swollen mandibular and superficial cervical lymph nodes. Fine-needle aspiration cytology of the superficial cervical lymph node revealed large lymphoblasts with mitoses present. The bovine leukemia virus (BLV) proviral load was relatively high, and phylogenetic analysis of the whole BLV genome classified the BLV strain as one with high viral replication activity. Genotyping of bovine leukocyte antigen genes indicated that the cow was susceptible to enzootic bovine leukosis (EBL). The bone morphogenetic protein 6 (BMP6) gene promoter region was hypermethylated. Monoclonal proliferation of B cells and monoclonal integration of the BLV provirus in the bovine genome were detected by a clonality test of B cells and an inverse PCR assay, respectively. At autopsy, generalized swelling of lymph nodes and spinal canal invasion by tumor tissue at vertebrae L5-6 were observed. Histologic analysis revealed diffuse proliferation of large round neoplastic cells that were positive for BLA36 and negative for CD3. The cow was definitively diagnosed with EBL based on these findings. Infection with a highly pathogenic strain of BLV, susceptibility of the BoLA-DRB3 alleles, and hypermethylation of the BMP6 gene may have contributed to the development of EBL in our case.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据