期刊
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
卷 16, 期 10, 页码 923-933出版社
WILEY
DOI: 10.1002/term.3340
关键词
amniotic fluid derived stromal cells (AFSC); angiogenesis; bone; bone marrow microenvironment; conditioned medium
类别
资金
- Korea Health Industry Development Institute [HI16C0797]
- National Research Foundation [2017R1A2B4005563, 2019R1A2C1085672]
- National Research Foundation of Korea [2019R1A2C1085672, 2017R1A2B4005563] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A study evaluated the effects of a conditioned medium of amniotic fluid-derived stromal cells (AFSC-CM) on bone metabolism. The results showed that AFSC-CM increased bone mass and enhanced bone turnover in mice. Additionally, AFSC-CM increased osteoclast differentiation and promoted endothelial cell migration and tube formation. The study suggests that AFSC-CM can have a bone anabolic effect by altering the bone marrow microenvironment, including angiogenesis and precursor expansion.
A cell-free approach utilizing the paracrine effects of mesenchymal stromal cells is receiving attention in regenerative medicine. In the present study, we evaluated the effects of a conditioned medium of amniotic fluid-derived stromal cells (AFSC-CM) on bone metabolism. In mice, intraperitoneal injections of AFSC-CM increased bone mass and enhanced bone turnover. The precursor populations of myeloid and mesenchymal lineages, as well as endothelial cells in bone marrow, were also augmented by AFSC-CM administration. In an in vitro culture experiment, AFSC-CM increased osteoclast differentiation of bone marrow-derived macrophages, but had no significant effect on the osteogenic differentiation of preosteoblasts. However, AFSC-CM administration dramatically accelerated the migration and tube formation of endothelial cells, and a cytokine array showed that AFSC-CM contained many angiogenic factors. These results indicate that AFSC-CM exerts a bone anabolic effect by changing the bone marrow microenvironment, including angiogenesis and precursor expansion. Therefore, ameliorating marrow angiogenesis is a potential therapeutic strategy for bone regeneration, for which AFSCs can be a good cellular source.
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