期刊
JOURNAL OF SEPARATION SCIENCE
卷 45, 期 15, 页码 2845-2854出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202200133
关键词
Bufo bufo gargarizans; Cinobufacini; mass spectrometry; peptidomics
资金
- Natural Science Foundation of China [82073975, 81673563, 81102762, 81274199]
- Open Project Program of Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization [ZDXM-2022-02, ZDXM-2020-18, FJGJS2015-15]
- Jiangsu Qing Lan Project for Young Academic Leaders of China
- Fund of Quality Standardization of Liu-ShenWan [BA2016104, ZYBZH-C-JS-30]
In this study, high-resolution mass spectrometry-based peptidomics platforms were used to identify and analyze peptides in Cinobufacini. Specific peptides were discovered using a gel- and HLB-based separation and purification system, and confirmed through target parallel reaction monitoring mode. This mapping provides important peptide information for the quality control of Bufo bufo gargarizans skin and its preparation.
Aqueous extract of toad skin (named as Cinobufacini or Huachansu) provides plentiful sources of bioactive peptides that remain undetected and unidentified. High-resolution mass spectrometry-based peptidomics platforms have developed into a major approach to the discovery of natural peptides, with data-dependent acquisition modes providing a wealth of peptide profiling information. In this study, we used a gel- and HLB (a solid phase extraction cartridge)-based two-dimensional separation and purification system and nanoliquid chromatography-tandem mass spectrometry-based peptidomic studies with homology matching for the identification of peptides from Cinobufacini. We evaluated 232 multi-charged peptides and found several specific peptides, some of which were validated by target parallel reaction monitoring mode. These peptides are the first to be identified in Cinobufacini and are completely different from ones identified in toad venom. So, this mapping provides key peptide information for the quality control of Bufo bufo gargarizans skin and its preparation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据