4.5 Article

Chemical profiling of composite prescription caraway and quantification of three pairs isomeric components in caraway administered rat plasma by tandem mass spectrometry

期刊

JOURNAL OF SEPARATION SCIENCE
卷 45, 期 16, 页码 3082-3094

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202200155

关键词

caraway; chemical profiling; quantification; rat plasma; tandem mass spectrometry

资金

  1. Institutes for Drug Discovery and Development, Chinese Academy of Sciences [CASIMM0620183001]
  2. Xinjiang Natural Science Foundation [2009211B34]

向作者/读者索取更多资源

Caraway, a traditional Uyghur medicine, has been used to treat vitiligo for centuries. In this study, the chemical components present in caraway were comprehensively characterized using ultra-high performance liquid chromatography coupled with hybrid quadrupole orbitrap mass spectrometry. Additionally, a tandem mass spectrometry method was developed and validated for quantitative analysis of specific components in rat plasma.
Caraway, a well-known traditional Uyghur medicine, has been used to treat vitiligo for centuries. Its biological effects on melanin synthesis of caraway have been investigated. However, beyond psoralen and isopsoralen alone, no further chemical component of caraway has been revealed. In this study, ultra-high performance liquid chromatography coupled with hybrid quadrupole orbitrap mass spectrometry was employed to comprehensively characterize the chemical components present in caraway. Based on accurate mass measurements, key fragmental ions and comparison with reference standards, 75 chemical components were identified in caraway. Moreover, a tandem mass spectrometry method was developed and validated for quantitative analysis of three pairs isomeric components, namely psoralen/isopsoralen, bavachin/isobavachalcone and bavachromene/isobavachromene in rat plasma. Psoralen, isopsoralen, bavachin, and isobavachalcone showed linearity with concentration ranging of 1.0-500.0 ng/ml. The linear ranges for bavachromene and isobavachromene were 0.2-500.0 ng/ml. The accuracies were in ranges of 85%-115% with coefficient of variation errors of less than 15%. Furthermore, the method was applied to quantify the three pairs isomeric components in rats after oral administration of caraway.

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