期刊
JOURNAL OF RHEUMATOLOGY
卷 50, 期 2, 页码 227-235出版社
J RHEUMATOL PUBL CO
DOI: 10.3899/jrheum.220160
关键词
extracellular vesicle; inflammation; juvenile arthritis; microRNA; synovial fluid; TGF-? superfamily proteins
类别
This study evaluated the expression of microRNAs in synovial fluid, plasma, and leukocytes from patients with juvenile idiopathic arthritis (JIA). The results showed dysregulated expression of multiple microRNAs in JIA, both locally and systemically. These findings provide important insights into the pathogenesis of JIA and may have implications for targeted therapies.
Objective. To evaluate microRNA expression in synovial fluid (SF), plasma, and leukocytes from patients with juvenile idiopathic arthritis (JIA).Methods. MicroRNA expression in pooled JIA plasma and SF was assessed by absolute quantitative droplet digital PCR array. The results were validated in individual patient samples. MicroRNA content in leukocytes and extracellular vesicles was evaluated by real-time PCR in JIA blood and SF. Blood microRNA expression was compared with healthy controls (HCs). Principal component analysis was used to profile JIA plasma and SF microRNAs, and the potential biological consequences of microRNA dysregulation were investigated by pathway analysis.Results. MiR-15a-5p and miR-409-3p levels were higher in JIA plasma than in HC plasma. JIA SF con-tained elevated levels of miR-21-5p, miR-27a-3p, miR-146b-5p, miR-155-5p, and miR-423-5p, and decreased miR-192-5p and miR-451a, compared to JIA plasma. Extracellular vesicle analysis demonstrated variable encapsulation among selected microRNAs, with only miR-155-5p being represented substantially in extracellular vesicles. SF leukocytes also had higher expression of miR-21-5p, miR-27a-3p, miR-146b-5p, and miR-155-5p, and lower expression of miR-409-3p and miR-451a, relative to blood. No differences were observed between JIA and HC blood leukocytes. Clusters of microRNAs were commonly altered in JIA joint fluid and leukocytes compared to JIA blood samples. In silico analysis predicted that differentially expressed microRNAs in JIA target the transforming growth factor (TGF)-beta pathway.Conclusion. The expression of multiple microRNAs is dysregulated in JIA both locally and systemically, which may inhibit the TGF-beta pathway. These findings advance our knowledge of JIA immunopathogenesis and may lead to the development of targeted therapies.
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