期刊
JOURNAL OF PHYSIOLOGY-LONDON
卷 601, 期 9, 页码 1675-1690出版社
WILEY
DOI: 10.1113/JP283299
关键词
mechanosensitive channels; myometrium; Piezo1; pregnancy; preterm labour; stretch-activated channels
This study identifies a mechanosensitive cation channel, Piezo1, in the uterine smooth muscle and microvascular endothelium of pregnant myometrium. Activation of Piezo1 relaxes the uterine tissue and its effects are associated with myocyte and endothelial nitric oxide signaling. These findings highlight the importance of stretch-activated channels in pregnancy maintenance and parturition.
Approximately 10% of US births deliver preterm before 37 weeks of completed gestation. Premature infants are at risk for life-long debilitating morbidities and death, and spontaneous preterm labour explains 50% of preterm births. In all cases existing treatments are ineffective, and none are FDA approved. The mechanisms that initiate preterm labour are not well understood but may result from dysfunctional regulation of quiescence mechanisms. Human pregnancy is accompanied by large increases in blood flow, and the uterus must enlarge by orders of magnitude to accommodate the growing fetus. This mechanical strain suggests that stretch-activated channels may constitute a mechanism to explain gestational quiescence. Here we identify for the first time that Piezo1, a mechanosensitive cation channel, is present in the uterine smooth muscle and microvascular endothelium of pregnant myometrium. Piezo is downregulated during preterm labour, and stimulation of myometrial Piezo1 in an organ bath with the agonist Yoda1 relaxes the tissue in a dose-dependent fashion. Further, stimulation of Piezo1 while inhibiting protein kinase A, AKT, or endothelial nitric oxide synthase mutes the negative inotropic effects of Piezo1 activation, intimating that actions on the myocyte and endothelial nitric oxide signalling contribute to Piezo1-mediated contractile dynamics. Taken together, these data highlight the importance of stretch-activated channels in pregnancy maintenance and parturition, and identify Piezo1 as a tocolytic target of interest.
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