期刊
JOURNAL OF PHYSICAL CHEMISTRY A
卷 126, 期 25, 页码 4036-4045出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jpca.2c02584
关键词
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资金
- US Department of Energy, Office of Science, Basic Energy Sciences [DE-SC0018902]
- U.S. Department of Energy (DOE) [DE-SC0018902] Funding Source: U.S. Department of Energy (DOE)
In this study, we investigated the conformational and isomeric populations in gas-phase protonated tripeptides using infrared predissociation spectroscopy. The results showed that the position of the methyl side-chain has a significant impact on the 3-D structure of the peptide, and the exact amino acid sequence determines the structural populations. These changes in structural populations can be explained by the electron-donating effect of the methyl side-chain.
We present a systematic study of the conformational and isomeric populations in gas-phase protonated tripeptides containing glycine and alanine residues using infrared predissociation spectroscopy of cryogenically cooled ions. Specifically, the protonated forms of GlyAla, Gly-Ala-Ala, and Ala-Ala-Ala allow us to sample all permutations of the methyl side-chain position, providing a comprehensive view of the effects of this simple side-chain on the 3-D structure of the peptide. The individual structural populations for all but one of these peptide species are determined via conformer-specific IR-IR double-resonance spectroscopy and comparison with electronic structure predictions. The observed structures can be classified into three main families defined by the protonation site and the number of internal hydrogen bonds. The relative contribution of each structural family is highly dependent on the exact amino acid sequence of the tripeptide. These observed changes in structural population can be rationalized in terms of the electron-donating effect of the methyl side-chain modulating the local proton affinities of the amine and various carbonyl groups in the tripeptide.
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