4.7 Article

Epigenetic disruption of placental genes by chronic maternal cafeteria diet in rats

期刊

JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 106, 期 -, 页码 -

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2022.109015

关键词

Cafeteria diet; Feto-placental development; Vascular Endothelial Growth Factor; Insulin-like Growth Factor 2; DNA Methylation

资金

  1. Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) [PITCT2018-01760]
  2. Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP 2015 11220150100338CO]
  3. Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral (FBCB, UNL, Santa Fe, Argentina)

向作者/读者索取更多资源

Maternal diet has a significant impact on reproduction, fetal development, and offspring behavior. This study demonstrates that chronic consumption of a Western-style diet impairs feto-placental development in female rats and may be attributed to epigenetic disruption of the Insulin-Like Growth Factor and Vascular Endothelial Growth Factor systems.
Maternal diet has impact on reproduction, fetal development and offspring behavior, although molecular mechanisms remained unknown. Our aims were to assess (1) the effects of a cafeteria (CAF) diet (western diet habits) on female reproductive performance, fetal and placental parameters on gestational day 21 and litter size and pup weight at birth; and (2) placental messenger RNA (mRNA) expression and epigenetic regulation of Insulin-Like Growth Factor (Igf) and Vascular Endothelial Growth Factor (Vegf) and their receptors. Female Wistar rats were fed with control or CAF diet from weaning until parturition. At week 14 after diets started, females were mated and half of the animals were euthanized on gestational day 21 to evaluate reproductive parameters including the pregnancy rate, number of corpora lutea, implantation sites and resorption sites. Moreover, fetal weight and length, placental weight, and placental index were recorded. Placentas were collected for mRNA quantification and DNA methylation analysis. The remaining animals were allowed to give birth and the number and weight of the pups were evaluated.CAF diet did not affect reproductive performance or fetal weight and length. However, CAF-fed animals showed a decrease in placental weight and index and the pups exhibited a low birth weight. Additionally, we found an upregulation of Igf2 and a down regulation of Vegf placental mRNA expression in CAF dams, associated with methylation status changes of their promoters. We conclude that female chronic CAF diet consumption impairs feto-placental development and could be explained by an epigenetic disruption of Igf and Vegf systems.(c) 2022 Published by Elsevier Inc.

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