期刊
JOURNAL OF MOLECULAR ENDOCRINOLOGY
卷 69, 期 2, 页码 315-327出版社
BIOSCIENTIFICA LTD
DOI: 10.1530/JME-21-0019
关键词
diabetic ulcers; wound healing; macrophages; M2 polarization; microRNA-146a; TLR4; NF-kappa B
资金
- Fujian Provincial Natural Science Foundation [2016J01611]
The study demonstrates that miR-146a can induce M2 macrophage polarization by inhibiting the TLR4/NF-kappa B axis, thereby enhancing wound healing in diabetic ulcers.
We tried to unveil the clinical significance of miR-146a as a biomarker in M2 macrophage polarization in diabetic wound healing. Initially, we found reduced miR-146a in macrophages of diabetic patients. Next, dual-luciferase assay verified that toll-like receptor 4 (TLR4) was a target gene of miR-146 and was negatively regulated by miR-146. Moreover, after ectopic expression and depletion experiments of miR-146 and/or TLR4, lipopolysaccharide-induced inflammatory response of macrophages was detected. The results revealed that overexpression of miR-146a promoted the M2 macrophage polarization by suppressing the TLR4/nuclear factor-kappaB (NF-kappa B) axis, so as to enhance wound healing in diabetic ulcers. Further, mouse models with diabetic ulcers were established to investigate the effects of miR-146a on diabetic wound healing in vivo, which revealed that miR-146a promoted wound healing in diabetic ulcers by inhibiting the TLR4/NF-kappa B axis. In conclusion, we demonstrate that miR-146a can induce M2 macrophage polarization to enhance wound healing in diabetic ulcers by inhibiting the TLR4/NF-kappa B axis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据