Engineering Toehold-Mediated Switches for Native RNA Detection and Regulation in Bacteria

RNA switches are versatile tools in synthetic biology for sensing and regulation applications. The discoveries of RNA-mediated translational and transcriptional control have facilitated the development of complex de novo designs of RNA switches. Specifically, RNA toehold-mediated switches, in which binding to the toehold sensing domain controls the transition between switch states via strand displacement, have been extensively adapted for coupling systems responses to specific trans-RNA inputs. This review highlights some of the challenges associated with applying these switches for native RNA detection in vivo, including transferability between organisms. The applicability and design considerations of toehold-mediated switches are discussed by highlighting twelve recently developed switch designs. This review finishes with future perspectives to address current gaps in the field, particularly regarding the power of structural prediction algorithms for improved in vivo functionality of RNA switches.(c) 2022 Elsevier Ltd. All rights reserved.

Automated summary

This article reviews the applications of RNA switches in synthetic biology, focusing on the design and considerations of toehold-mediated switches. It also discusses the challenges of applying these switches for native RNA detection in vivo and offers future research perspectives.