Advances in three-dimensional bioprinted stem cell-based tissue engineering for cardiovascular regeneration

Three-dimensional (3D) bioprinting of cellular or biological components are an emerging field to develop tissue structures that mimic the spatial, mechanochemical and temporal characteristics of cardiovascular tissues. 3D multi-cellular and multi-domain organotypic biological constructs can better recapitulate in vivo physiology and can be utilized in a variety of applications. Such applications include in vitro cellular studies, high-throughput drug screening, disease modeling, biocompatibility analysis, drug testing and regenerative medicine. A major challenge of 3D bioprinting strategies is the inability of matrix molecules to reconstitute the complexity of the extracellular matrix and the intrinsic cellular morphologies and functions. An important factor is the inclusion of a vascular network to facilitate oxygen and nutrient perfusion in scalable and patterned 3D bioprinted tissues to promote cell viability and functionality. In this review, we summarize the new generation of 3D bioprinting techniques, the kinds of bioinks and printing materials employed for 3D bioprinting, along with the current stateof-the-art in engineered cardiovascular tissue models. We also highlight the translational applications of 3D bioprinting in engineering the myocardium cardiac valves, and vascular grafts. Finally, we discuss current challenges and perspectives of designing effective 3D bioprinted constructs with native vasculature, architecture and functionality for clinical translation and cardiovascular regeneration.

Automated summary

Three-dimensional bioprinting is an emerging field that aims to develop tissue structures mimicking the characteristics of cardiovascular tissues. These structures have various applications including cellular studies, drug screening, disease modeling, and regenerative medicine. However, the complexity of the extracellular matrix and cellular morphologies and functions pose challenges in 3D bioprinting. This review summarizes the latest techniques, bioinks, and printing materials used for 3D bioprinting, as well as the current state of engineered cardiovascular tissue models. The review also discusses challenges and perspectives in designing effective 3D bioprinted constructs for clinical translation and cardiovascular regeneration.