期刊
JOURNAL OF MICROBIOLOGICAL METHODS
卷 198, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.mimet.2022.106494
关键词
Acidithiobacillus; Acidithiobacillus thiooxidans; Bioleaching; Culture growth; High-metal-content residues
资金
- Consejo Nacional de Ciencia y Tecnologia [131203]
This study proposes a DNA quantification technique to evaluate the growth of Acidithiobacillus thiooxidans, which remains effective even in the presence of high-metal-content residues. This method could serve as a useful tool for evaluating Acidithiobacillus growth and developing biometallurgical applications.
The potential of Acidithiobacillus (Thiobacillus) genus members, namely Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, for bioleaching purposes is known. Specifically, previous studies have shown the potential of A. thiooxidans strain DSM 26636 used in bioleaching processes to remove metals in high-metal-content matrices. All Acidithiobacillus growth-monitoring techniques available to date, including sulfate production, commonly used, present disadvantages. Thus, the current work shows a technique based on DNA quantification to evaluate the growth of A. thiooxidans DSM 26636, which is useful even in the presence of a high-metal-content residue. This proposed methodology may represent a functional complementary tool to evaluate Acidithiobacillus growth to develop biometallurgical applications.
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