期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 233, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2022.111843
关键词
Acylthiourea ligands; Palladium(II) complexes; Biomolecular interactions; Apoptosis; Antioxidant; Hemolysis
资金
- Department of Science and Technology, Ministry of Science and Technology, Government of India [IF170457]
- Fondo Nacional de Ciencia y Tecnologia (FONDECYT) [3200391, 11170840]
- Ministry of Science and Technology, Taiwan [MOST108-2113-M-037-016, MOST109-2314B-037-069-MY3]
A series of Pd(II) complexes based on acylthiourea ligands were synthesized and characterized. Complex 4 exhibited good activity against cancer cells, inducing apoptosis. Furthermore, complex 4 showed high scavenging activity against free radicals and biocompatibility with red blood cells.
Acylthiourea-based Pd(II) complexes (1-5) with a PPh3 moiety bearing the general formula [PdCl(PPh3)(L-R)] [L-R = monoanionic bidentate acylthiourea ligand, where R = C6H5 (L1), C6H4CH3(o) (L2), C6H4OCH2CH3(p) (L3), C10H7 (L4) or C6H4Cl (L5)] have been synthesized and characterized by spectroscopic and analytical tools. The single crystal X-ray structures (1-3) revealed that the acylthiourea ligands coordinated to Pd(II) ion in an uncommon bidentate fashion through S and N atoms, forming a four-member ring. The Pd(II) ion exhibited a square planar geometry fulfilled by the ligand (N, S), one Cl- and one triphenylphosphine (PPh3). Calf thymus (CT) DNA and bovine serum albumin (BSA) binding of the complexes have been analyzed by spectroscopic and molecular docking studies. The complexes were tested for their in vitro cytotoxicity on three cancer (cervical, breast and lung) and one normal (human embryo) cell lines. Complex 4 bearing the naphthalene substitution exhibited the highest activity against three cancer cells with the half-maximal inhibitory concentration (IC50) values of 8.6 (cervical), 8.8 (breast) and 9.4 mu M (lung). The acridine orange/ethidium bromide (AO/EB) and 4',6-diamidino-2-phenylindole (DAPI) staining assays indicated that 4 induced cancer cell death through apoptosis. Among the complexes, 4 exhibited the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of 86.19%. All the complexes were subjected to the hemolysis assay which revealed their biocompatibility with red blood cells (RBCs) with a lysis rate of less than 5 %.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据