期刊
JOURNAL OF CONTROLLED RELEASE
卷 223, 期 -, 页码 53-63出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2015.12.020
关键词
SiRNA; Chitosan; RGD peptidomimetic; Tumor metabolism; Lactate; Glutamine
资金
- Fonds national de la Recherche Scientifique (FRS-FNRS)
- Belgian Foundation Against Cancer
- J. Maisin Foundation
- Belgian Science Policy Office (Belspo) [UP7-03]
- Action de Recherche Concertee [ARC 14/19-058]
- Walloon Region (BioWin project Targetum)
PEGylated chitosan-based nanoparticles offer attractive platforms for siRNA cocktail delivery into tumors. Still, therapeutic efficacy requires us to select a rational combination of siRNAs and an efficient tumor delivery after systemic administration. Here, we showed that non-covalent PEGylation of chitosan-based nanoparticles loaded with siRNA targeting two key transporters of energy fuels for cancer cells, namely the lactate transporter MCT1 and the glutamine transporter ASCT2, could lead to significant antitumor effects. As a ligand, we tested variations of the prototypical RGD peptidomimetic (RGDp). A higher siRNA delivery was obtained with naphthyridine-containing RGDp randomly conjugated on the PEG chain by clip photochemistry and the use of a lipophilic linker than when using traditional chain-end grafting and RGDp with a hydrophilic linker. The antiproliferative effects resulting from ASCT2 and MCT1 silencing were validated separately in vitro in conditions mimicking specific metabolic profiles of cancer cells and in vivo upon concomitant delivery. The combination of those siRNA and the selected components of targeted RGDp nanoparticles led to a dramatic tumor growth inhibition upon peri-tumoral but also systemic administration in mice. Altogether these data emphasize the convenience of using non-covalent PEGylated chitosan particles to produce sheddable stealth protection compatible with an efficient siRNA delivery in tumors. (C) 2015 Elsevier B.V. All rights reserved.
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