期刊
JOURNAL OF GENERAL VIROLOGY
卷 103, 期 7, 页码 -出版社
MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.001767
关键词
Borna disease virus; matrix protein; glycoprotein; reverse genetics; virus particle production
资金
- JSPS KAKENHI [JP19J23468, JP19K22530, JP20H05682, JP21K19909]
- MEXT KAKENHI [JP16H06429, JP16K21723, JP16H06430]
- JSPS Core-to-Core Program
- Joint Usage/Research Center Program on Institute for Life and Medical Sciences, Kyoto University
The study found that exogenous expression of Matrix protein (M) and Glycoprotein (G), which are constituents of the viral lipid envelope, significantly facilitates the formation and propagation of BoDV-1 infectious particles without affecting viral RNA synthesis. Additionally, simultaneous transfection of M and G expression plasmids with N, P, and L helper plasmids improves the rescue efficiency of recombinant BoDV-1. The findings suggest that BoDV-1 may suppress excess expression of M and G to reduce cytopathic effects, leading to persistent infection.
Borna disease virus 1 (BoDV- 1) is a non-segmented, negative-strand RNA virus that is characterized by persistent infection in the nucleus and low production of progeny virions. This feature impedes not only the harvesting of infectious viral particles from infected cells but also the rescue of high titres of recombinant BoDV- 1 (rBoDV- 1) by reverse genetics. Here, we dem-onstrate that exogenous expression of both matrix protein (M) and glycoprotein (G), which are constituents of the viral lipid envelope, significantly facilitates the formation of infectious particles and propagation of BoDV- 1 without affecting its viral RNA synthesis. Furthermore, simultaneous transfection of M and G expression plasmids with N, P and L helper plasmids by reverse genetics drastically enhances the rescue efficiency of rBoDV- 1. On the other hand, we also show that overexpression of M induces obvious cytotoxicity similar to that of other Mononegaviruses. Together with our recent report showing that excess expression of G induces aberrant accumulation of immature G, a potential stimulator of the host innate immune response, it is conceivable that BoDV- 1 may suppress excess expression of M and G to reduce the cytopathic effect, thereby leading to main-tenance of persistent infection. Our results contribute not only to the establishment of an efficient method to recover high -titre BoDV- 1 but also to understanding the unique mechanism of persistent BoDV- 1 infection.
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