Metagenomic and physicochemical analysis of Kombucha beverage produced from tea waste

Kombucha beverage produced through fermentation of sugared tea using bacteria and yeast has gained attention for its beneficial health benefits. However, the cost linked to the raw materials often increases the upstream process expenses, thereby the overall operating expenditures. Thus, there is a need to explore alternative waste and cost-effective raw materials for Kombucha fermentation. The present study, compared the physico-chemical and microbial growth pattern of Kombucha beverage production using tea waste from the tea processing industries with that of the green/black tea, reporting similar trends irrespective of its type. Further, the amplicon sequencing of 16S rRNA showed dominant presence of Komagataeibacter rhaeticus and high throughput sequencing of ITS1 confirmed the presence of yeast species similar to Brettanomyces bruxellensis in the tea waste based Kombucha beverage. Appreciable amount of carbohydrates (8.5/100 g) and energy (34 kcal/100 g) with appropriate organoleptic properties favourable for human consumption were also observed during the nutritional content and qualitative property assessment. The overall study showed a broad taxonomic and functional diversity existing during Kombucha fermentation process with tea waste to maintain a sustained eco-system to facilitate cost-effective beverage production with desired properties for safe consumption.

Automated summary

This study compared the physico-chemical and microbial growth patterns of Kombucha beverage production using tea waste from the tea processing industries with that of green/black tea, and found similar trends irrespective of the type of tea used. The Kombucha beverage made from tea waste contained a dominant presence of Komagataeibacter rhaeticus and yeast species similar to Brettanomyces bruxellensis. The nutritional content and qualitative properties of the tea waste-based Kombucha beverage were also suitable for human consumption.