Insight into multi-scale structural evolution during gelatinization process of normal and waxy maize starch

By using a mimicked heating with ex-situ liquid nitrogen flash freezing method, multi-scale structural evolution behaviors of normal maize starch (NMS) and waxy maize starch (WMS) during gelatinization process were studied. The results from SEM, X-ray diffraction (XRD), FTIR/solid state NMR spectroscopy and small angle X-ray scattering (SAXS) showed that NMS and WMS exhibited differently structural evolution behavior during gelatinization process. As the temperature increase, the proportion of the NMS granules with cavity gradually increased, while after heating beyond (peak gelatinization temperature (Tp) + conclusion gelatinization temperature (Tc))/2 the disappearance of starch granule integrity occurred for WMS. The relative crystallinity of NMS declined from 32.8 to 15.26% gradually, as that of WMS declined from 42.43 to 13.09% with a sharply descent when heated beyond (Tp + Tc)/2. The loss of short-range order structure of NMS and WMS showed same trends according to FTIR and NMR. Semicrystalline lamellae of NMS became thinner gradually while that of WMS showed an apparently narrowing after heating beyond (Tp + Tc)/2. These results suggest that the destruction of double helix in amylopectin structure had greatly influence on the larger scale structure of starch samples. This strategy is important for thorough understanding and profiting starch-based food processing.

Automated summary

Through a mimicked heating and ex-situ liquid nitrogen flash freezing method, it was found that normal maize starch and waxy maize starch exhibited different structural evolution behaviors during the gelatinization process. The proportion of cavities in normal maize starch granules gradually increased with temperature, while the integrity of starch granules in waxy maize starch disappeared after heating beyond half of the peak gelatinization temperature. Furthermore, the relative crystallinity of both starches decreased gradually with increasing temperature.