期刊
JOURNAL OF FOOD BIOCHEMISTRY
卷 46, 期 10, 页码 -出版社
WILEY-HINDAWI
DOI: 10.1111/jfbc.14351
关键词
AMPK/SIRT1/NF-kappa B signaling pathway; heart failure; inflammation; liquiritin; oxidative stress
资金
- National Natural Science Foundation of China [81373533, 81973844, 82004180]
- Key Project Foundation of Natural Science Research in Universities of Anhui Province in China [KJ2020A0393]
- Key Project Foundation of Natural Science Research of Anhui University of Chinese Medicine [2020zrzd06]
Liquiritin, the main ingredient of licorice, has antioxidant and anti-inflammatory properties and can alleviate oxidative stress and inflammation in cardiomyocytes by regulating the AMPK/SIRT1/NF-κB signaling pathway.
Heart failure (HF) is a serious disease with high mortality. Oxidative stress plays a vital role in its occurrence and development. Licorice is commonly used to treat HF in traditional Chinese medicine. Liquiritin, the main ingredient of licorice, has antioxidant and anti-inflammatory properties, but the mechanism against oxidative stress in cardiomyocytes has not been reported. Establishment of oxidative damage model in H9c2 cells by hydrogen peroxide (H2O2). Liquiritin (5, 10, 20 mu mol/L) could significantly prevent the loss of cell viability and decrease the apoptosis rate. It can reduce the levels of reactive oxygen species (ROS), malonedialdehyde (MDA), lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and increase the activity of ATP, superoxidedismutase (SOD), glutathione peroxide (GSH-px), glutathione reductase (GR) and catalase (CAT) to alleviate oxidative stress and inflammation in a dose-dependent manner. Liquiritin was found to be related to AMP-Activated Protein Kinase (AMPK) pathway by molecular docking. Western blotting (WB) and quantitative reverse transcription PCR (RT-qPCR) confirmed that liquiritin could promote AMPK alpha phosphorylation and sirtuin 1 (SIRT1) protein expression, and inhibit phosphorylation of nuclear factor kappa B p65 (NF-kappa B p65). Compound C, EX 527, and PDTC can reverse the effects of liquiritin, indicating that its antioxidant effect is achieved by regulating AMPK/SIRT1/NF-kappa B signaling pathway.
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