期刊
JOURNAL OF DAIRY SCIENCE
卷 105, 期 9, 页码 7242-7252出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2021-21082
关键词
raw milk adulteration; Raman spectroscopy; PLS-DA; PLS
资金
- Science and Tech- nology Innovation Action Plan?
- Science and Technology Innovation Action Plan Agricultural Project of Shanghai Science and Technology Commission [19391902600]
- Shanghai Engineering Technology Research Center of Shanghai Science and Technology Commissio [20DZ2255600]
In this study, Raman spectroscopy combined with chemometrics was successfully used for rapid on-site identification and quantification of common adulterants in raw milk. The method of Raman spectroscopy combined with PLS-DA and PLS model can accurately detect maltodextrin, sodium carbonate, and whey adulteration, and has high classification and detection accuracy.
To achieve rapid on-site identification of raw milk adulteration and simultaneously quantify the levels of various adulterants, we combined Raman spectroscopy with chemometrics to detect 3 of the most common adulterants. Raw milk was artificially adulterated with maltodextrin (0.5-15.0%; wt/wt), sodium carbonate (10-100 mg/kg), or whey (1.0-20.0%; wt/wt). Partial least square discriminant analysis (PLS-DA) classifica-tion and a partial least square (PLS) regression model were established using Raman spectra of 144 samples, among which 108 samples were used for training and 36 were used for validation. A model with excellent per-formance was obtained by spectral preprocessing with first derivative, and variable selection optimization with variable importance in the projection. The clas-sification accuracy of the PLS-DA model was 95.83% for maltodextrin, 100% for sodium carbonate, 95.84% for whey, and 92.25% for pure raw milk. The PLS model had a detection limit of 1.46% for maltodextrin, 4.38 mg/kg for sodium carbonate, and 2.64% for whey. These results suggested that Raman spectroscopy com-bined with PLS-DA and PLS model can rapidly and efficiently detect adulterants of maltodextrin, sodium carbonate, and whey in raw milk.
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