期刊
JOURNAL OF CELL SCIENCE
卷 135, 期 17, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.259658
关键词
Deltex; Dpp; Tkv; Endocytosis; Morphogen gradient; Drosophila
类别
资金
- Council of Scientific and Industrial Research (CSIR), Government of India
- Department of Science and Technology, Ministry of Science and Technology, India [CRG/2021/006975]
- Institute of Eminence Scheme, Banaras Hindu University, India
Dx is a regulator of Notch signaling that can facilitate the endocytosis of the Notch receptor. In this study, we identified Dx as a novel modulator of Dpp signaling and demonstrated its genetic interaction with dpp and its pathway components. Immunocytochemical analysis showed that Dx colocalizes with Dpp and its receptor Tkv in Drosophila larval tissues. Furthermore, we found that Dx modulates the expression of dpp and its target genes, suggesting its involvement in the endocytosis and trafficking of Dpp.
Deltex (Dx) is a context-dependent regulator of Notch signaling that can act in a non-canonical fashion by facilitating the endocytosis of the Notch receptor. In an RNAi-based modifier screen of kinases and phosphatases, we identified Thickveins (Tkv), the receptor of Decapentaplegic (Dpp), as one of the interactors of Dx. Dpp, a Drosophila homolog of TGF-beta and bone morphogenetic proteins, acts as a morphogen to specify cell fate along the anterior-posterior axis of the wing. Tight regulation of Dpp signaling is thus indispensable for its proper functioning. Here, we present Dx as a novel modulator of Dpp signaling. We show evidence for the very first time that dx genetically interacts with dpp and its pathway components. Immunocytochemical analysis revealed that Dx colocalizes with Dpp and its receptor Tkv in Drosophila third-instar larval tissues. Furthermore, Dxwas also seen to modulate the expression of dpp and its target genes, and we attribute this modulation to the involvement of Dx in the endocytosis and trafficking of Dpp. This study thus presents awhole newavenue of Dpp signaling regulation via the cytoplasmic protein Dx.
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