4.6 Article

Miniaturized optimal incident light angle-fitted dark field system for contrast-enhanced real-time monitoring of 2D/3D-projected cell motions

期刊

JOURNAL OF BIOPHOTONICS
卷 15, 期 10, 页码 -

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.202200091

关键词

3D projection; dark field filter; dark field microscope; light incident angle; real-time cell monitoring

资金

  1. Ministry of Science and ICT [2019R1I1A3A01060695]
  2. National Research Foundation (NRF) of Korea [NRF2020R1A4A2002817]
  3. National Research Foundation of Korea [2019R1I1A3A01060695] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

向作者/读者索取更多资源

Dark field microscopy provides better insight into biology, but most microscopes are limited to 2D samples. We propose a compact cell monitoring system with an optimized dark field filter to enable real-time cell imaging and long-term monitoring.
In the field of biology, dark field microscopy provides superior insight into cells and subcellular structures. However, most dark field microscopes are equipped with a dark field filter and a light source on a 2D-based specimen, so only a flat sample can be observed in a limited space. We propose a compact cell monitoring system with built-in dark field filter with an optimized incident angle of the light source to provide real-time cell imaging and spatial cell monitoring for long-term free from phototoxicity. 2D projection imaging was implemented using a modular condenser lens to acquire high-contrast images. This enabled the long-term monitoring of cells, and the real-time monitoring of cell division and death. This system was able to image, by 2D projection, cells on the surface thinly coated with multiwalled carbon nanotubes, as well as living cells that migrated along the surface of glass beads and hydrogel droplets with a diameter of about 160 mu m. The optimal incident light angle-fitted dark field system combines high-contrast imaging sensitivity and high spatial resolution to even image cells on 3D surfaces.

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