期刊
JOURNAL OF APPLIED MICROBIOLOGY
卷 133, 期 5, 页码 3030-3040出版社
WILEY
DOI: 10.1111/jam.15764
关键词
antibacterial activity; Halobacillus; metabolites; pyrrole; reactive oxygen species
资金
- 2020 Li Ka Shing Foundation (LKSF) Cross-Disciplinary Research Grant [2020LKSFG07A]
- Guangdong Basic and Applied Basic Research Foundation [2021A1515110426]
- Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) [GML2019ZD0606]
- National Natural Science Foundation of China [92051118, 32070113]
- Scientific Research Foundation of Shantou University [NTF21030]
This study successfully isolated antimicrobial compounds from H. marinus HMALI004, which can inhibit the growth of opportunistic marine pathogens V. parahaemolyticus and V. cholerae by increasing reactive oxygen species.
Aims This study examined and characterized the extract for metabolites of Halobacillus marinus HMALI004 to understand their antibacterial activities against opportunistic marine pathogens, that is, Vibrio parahaemolyticus and Vibrio cholerae. Methods and Results The bacterial strain HMALI004 was characterized as H. marinus, and an antibacterial spectral test revealed its inhibition against two opportunistic marine pathogens (V. parahaemolyticus and V. cholera). Fermentation broth of strain HMALI004 was subjected to column chromatography and high-performance liquid chromatography to separate antibacterial substances. Two compounds were successfully isolated and identified as 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid by mass spectrometry (MS) and nuclear magnetic resonance. The minimal inhibition concentration (MIC) values of 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid for V. parahaemolyticus were 25 mu g/ml, while their MIC values for V. cholerae were 50 and 100 mu g/ml, respectively. The reactive oxygen species (ROS) production of two pathogen strains treated with 1H-pyrrole-2-carboxylic acid and 4-chloro-1H-pyrrole-2-carboxylic acid were detected to investigate the antimicrobial mechanism. The results suggested that 4-chloro-1H-pyrrole-2-carboxylic acid exerted enhanced ROS production in V. parahaemolyticus, whereas 1H-pyrrole-2-carboxylic acid had a weaker effect. Both compounds caused a significant rise in ROS production in V. cholerae, causing severe damage to the cell wall and cytoplasm, leading to cell death. Conclusions The bacterium H. marinus HMALI004 was isolated from a shrimp pond and was found to produce antimicrobial compounds, which could inhibit the growth of opportunistic marine pathogens V. parahaemolyticus and V. cholerae by increasing ROS. Significance and Impact of the Study Successfully isolated antibacterial-producing strain, H. marinus HMALI004, and its antimicrobial compounds could be used as biological control agents for marine pathogens.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据