期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 151, 期 2, 页码 526-+出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2022.07.022
关键词
Neutrophilic asthma; PD-1 agonist; T cells; lung inflammation; humanized mice
PD-1 plays a crucial role in regulating acute neutrophilic inflammation, and its deficiency is associated with increased airway hyperreactivity and lung inflammation. Conversely, PD-1 agonist treatment can effectively alleviate these pathological responses.
Background: Neutrophilic asthma is associated with disease severity and corticosteroid insensitivity. Novel therapies are required to manage this life-threatening asthma phenotype. Programmed cell death protein-1 (PD-1) is a key homeostatic modulator of the immune response for T-cell effector functions. Objective: We sought to investigate the role of PD-1 in the regulation of acute neutrophilic inflammation in a murine model of airway hyperreactivity (AHR). Methods: House dust mite was used to induce and compare neutrophilic AHR in wild-type and PD-1 knockout mice. Then, the therapeutic potential of a human PD-1 agonist was tested in a humanized mouse model in which the PD-1 extracellular domain is entirely humanized. Single-cell RNA sequencing and flow cytometry were mainly used to investigate molecular and cellular mechanisms. Results: PD-1 was highly induced on pulmonary T cells in our inflammatory model. PD-1 deficiency was associated with an increased neutrophilic AHR and high recruitment of inflammatory cells to the lungs. Consistently, PD-1 agonist treatment dampened AHR, decreased neutrophil recruitment, and modulated cytokine production in a humanized PD-1 mouse model. Mechanistically, we demonstrated at the transcriptional and protein levels that the inhibitory effect of PD-1 agonist is associated with the reprogramming of pulmonary effector T cells that showed decreased number and activation. Conclusions: PD-1 agonist treatment is efficient in dampening neutrophilic AHR and lung inflammation in a preclinical humanized mouse model.
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