期刊
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
卷 150, 期 5, 页码 1194-1208出版社
MOSBY-ELSEVIER
DOI: 10.1016/j.jaci.2022.05.025
关键词
Food allergy; skin dendritic cells; allergen immunotherapy; epicutaneous immunotherapy; T-cell responses; desensitization
资金
- Institut National de la Sante et de la Recherche Medicale (Inserm)
- DBV Technologies
This study investigates the phenotype and functional characteristics of skin dendritic cells (skDCs) during epicutaneous immunotherapy (EPIT), finding that skDCs gradually change their phenotype and functional properties during EPIT, which is crucial for achieving desensitization.
Background: Epicutaneous immunotherapy (EPIT) protocols have recently been developed to restore tolerance in patients with food allergy. The mechanisms by which EPIT protocols promote desensitization rely on a profound immune deviation of pathogenic T-and B-cell responses. Objective: To date, little is known about the contribution of skin dendritic cells (skDCs) to T-cell remodeling and EPIT efficacy. Methods: We capitalized on a preclinical model of food allergy to ovalbumin (OVA) to characterize the phenotype and functions of OVA(+) skDCs throughout the course of EPIT. Results: Our results showed that both Langerhans cells and dermal conventional cDC1 and cDC2 subsets retained their ability to capture OVA in the skin and to migrate toward the skin-draining lymph nodes during EPIT. However, their activation/maturation status was significantly impaired, as evidenced by the gradual and selective reduction of CD86, CD40, and OVA protein expression in respective subsets. Phenotypic changes during EPIT were also characterized by a progressive diversification of single-cell gene signatures within each DC subset. Interestingly, we observed that OVA(+) Langerhans cells progressively lost their capacity to prime CD4(+) T-EFF cells, but gained regulatory T-cell stimulatory properties. In contrast, cDC1 were inefficient in priming CD4(+) T-EFF cells or in reactivating T-MEM cells in vitro, whereas cDC2 retained moderate stimulatory properties, and progressively biased type 2 immunity toward type 1 and type 17 responses. Conclusions: Our results therefore emphasize that the acquisition of distinct phenotypic and functional specializations by skDCs during EPIT is at the cornerstone of the desensitization process.
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