期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 70, 期 29, 页码 9095-9105出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.2c02949
关键词
Amadori rearrangement product; glutathione; cysteine; pyrazines; sulfur-containing volatile compounds; furans
资金
- National Natural Science Foundation of China [32172330, JUFSTR20180204]
- Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province
- National Natural Science Foundation of China [32172330]
- National First-Class Discipline Program of Food Science and Technology [JUFSTR20180204]
- Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province
An Amadori rearrangement product derived from ribose and glutathione was studied, and it was found that higher temperatures accelerate its degradation and favor the formation of certain compounds. Additionally, extra-added glutathione or its constituent amino acids promote the generation of volatile compounds, with cysteine being the most effective in producing sulfur-containing volatiles.
An Amadori rearrangement product (ARP) derived from ribose (Rib) and glutathione (GSH) was prepared and identified as N-(1-deoxy-D-ribulos-1-yl)-glutathione by ultraperformance liquid chromatography-tandem mass spectrometry and NMR. Thermal treatment of the ARP aqueous solution was conducted, and a relatively high temperature was found to accelerate the degradation of the ARP. The concentration of furans formed at 120 degrees C was more than 6.39 times that at 100 degrees C, and especially, the high temperature favored the formation of furfural and 4-hydroxy-5-methyl-3(2H)-furanone through deoxyosone dehydration. The promoting role of extra-added GSH or its constituent amino acids was investigated in the volatile formation during thermal processing of the ARP. Both, the added GSH and its constituent amino acids, could timely capture glyoxal (GO) and methylglyoxal (MGO) to facilitate Strecker degradation, which improved pyrazine formation. Compared with glycine and glutamic acid, cysteine was the most effective extra-added amino acid to react with GO and MGO to produce pyrazine and methylpyrazine. More importantly, the cysteine degraded from extra-added GSH effectively accelerated the generation of sulfur-containing volatile compounds through the reaction of cysteine degradation products with furans and shorter-chain alpha-dicarbonyl compounds.
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