4.7 Article

Epigenetic and Transcriptomic Programming of HSC Quiescence Signaling in Large for Gestational Age Neonates

期刊

出版社

MDPI
DOI: 10.3390/ijms23137323

关键词

epigenomics; single-cell; stem-cells; fetal programming; hematopoiesis

资金

  1. Roadmap Epigenomics Program [R01 HD063791]
  2. Einstein Center for Epigenomics
  3. Einstein Epigenomics Shared Facility
  4. Einstein Computational Epigenomics Group

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This study reveals that excessive fetal growth leads to DNA methylation alterations and chromatin rearrangements in hematopoietic stem cells, which in turn affect the functions and quiescence signaling of these cells. This finding provides insights into the potential negative impact of fetal overgrowth on health.
Excessive fetal growth is associated with DNA methylation alterations in human hematopoietic stem and progenitor cells (HSPC), but their functional impact remains elusive. We implemented an integrative analysis combining single-cell epigenomics, single-cell transcriptomics, and in vitro analyses to functionally link DNA methylation changes to putative alterations of HSPC functions. We showed in hematopoietic stem cells (HSC) from large for gestational age neonates that both DNA hypermethylation and chromatin rearrangements target a specific network of transcription factors known to sustain stem cell quiescence. In parallel, we found a decreased expression of key genes regulating HSC differentiation including EGR1, KLF2, SOCS3, and JUNB. Our functional analyses showed that this epigenetic programming was associated with a decreased ability for HSCs to remain quiescent. Taken together, our multimodal approach using single-cell (epi)genomics showed that human fetal overgrowth affects hematopoietic stem cells' quiescence signaling via epigenetic programming.

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