期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/ijms23147668
关键词
extracellular vesicles; platelet lysate; in vitro wound healing; hyaluronic acid; regeneration; gingival fibroblasts; gingival keratinocytes
资金
- Instituto de Salud Carlos III
- ERDF European Regional Development Fund [MS16/00124, FI18/00104, PI17/01605, PI20/00127]
- Direccio General d'Investigacio, Conselleria d'Investigacio, Govern Balear [FPI/2046/2017]
- Institut d'Investigacio Sanitaria de les Illes Balears [ITS2018-002-TALENT, JUNIOR18/01]
- Health Research Institute of the Balearic Islands (IdISBa)
- ESF European Social Fund
This study investigated the combination of extracellular vesicles (EVs) derived from platelet lysate (PL) and hyaluronic acid biomaterials (HA) in an in vitro wound healing assay. The results showed that the combination of EVs and HA had good biocompatibility in gingival fibroblasts and keratinocytes, and could enhance wound healing in vitro. Furthermore, changes in gene expression related to extracellular matrix remodeling were observed after treatment with EVs.
Gingival regeneration aims at restoring the architecture and functionality of oral damaged tissue. Different biomaterials or biological materials have been tested for tissue repair, such as platelet concentrates such as PL. In this article, the use of extracellular vesicles (EVs) derived from platelet lysate (PL) and their combination with hyaluronic acid biomaterials (HA) in an in vitro wound healing assay is investigated. EVs were isolated by size exclusion chromatography from PL. In addition, HA gels were formulated with PL or EVs. EVs or HA combined with EVs (HA-EVs) were tested in vitro in gingival fibroblasts and keratinocytes for biocompatibility (LDH activity and metabolic activity) and by an in vitro wound-healing assay and gene expression analysis. EVs and EVs-HA treatments were biocompatible in gingival fibroblasts and keratinocytes and showed an increase in wound healing in vitro compared to control. Moreover, changes in gene expression related to extracellular matrix remodeling were observed after the treatment with EVs. EVs can be combined with HA biomaterials, showing good biocompatibility and preserving their activity and functionality. Therefore, platelet-derived EVs could emerge as a new application for periodontal regeneration in combination with biomaterials in order to enhance their clinical use.
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