4.7 Article

Enhanced Muscle Fibers of Epinephelus coioides by Myostatin Autologous Nucleic Acid Vaccine

期刊

出版社

MDPI
DOI: 10.3390/ijms23136997

关键词

Epinephelus coioides; myostatin; nucleic acid vaccine; skeletal muscle

资金

  1. Provincial Marine six Industries Special project for Promoting High-quality Economic Development (Marine economic development) - Department of Natural Resources of Guangdong Province [GDNRC[2022]50]
  2. Guangdong Forestry Science and Technology Innovation Project [2021KJCX012, 2022KJCX019]
  3. Provincial Science and Technology Special Fund Project for Zhongshan City (major special project + Task list management mode) [2021sdr003]
  4. Rural Science and Technology Commissioner Service Team project of New Rural Development Institute, South China Agricultural University [2021XNYNYKJHZGJ022]
  5. Provincial Projects with Special Funds for Promoting Economic Development of Marine and Fisheries Department of Guangdong [SDYY-2018-05]

向作者/读者索取更多资源

This study investigated the role of myostatin in E. coioides and demonstrated the positive effects of autologous nucleic acid vaccine on muscle growth.
Epinephelus coioides is a fish species with high economic value due to its delicious meat, high protein content, and rich fatty acid nutrition. It has become a high-economic fish in southern parts of China and some other Southeast Asian countries. In this study, the myostatin nucleic acid vaccine was constructed and used to immunize E. coioides. The results from body length and weight measurements indicated the myostatin nucleic acid vaccine promoted E. coioides growth performance by increasing muscle fiber size. The results from RT-qPCR analysis showed that myostatin nucleic acid vaccine upregulated the expression of myod, myog and p21 mRNA, downregulated the expression of smad3 and mrf4 mRNA. This preliminary study is the first report that explored the role of myostatin in E. coioides and showed positive effects of autologous nucleic acid vaccine on the muscle growth of E. coioides. Further experiments with increased numbers of animals and different doses are needed for its application to E. coiodes aquaculture production.

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