Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of Sechium edule Roots

Chayote (Sechium edule) produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. 'Tuershao', a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization.

Automated summary

This study investigated the molecular mechanism of chayote tuber formation through integrative analysis of metabolome and transcriptome profiles at different stages of tuber growth. The study revealed that genes and metabolites related to starch biosynthesis and galactose metabolism were upregulated during tuber bulking, while sugar transporter genes were highly expressed during tuber formation. The upregulation of auxin and ethylene precursors indicated the important roles of these hormones in tuber development and maturation. The study also identified a similar signaling pathway and active cell division during the initial stages of tuber formation in chayote as in potatoes.