期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 15, 页码 -出版社
MDPI
DOI: 10.3390/ijms23158817
关键词
hyaluronic acid; molecular weight; tendon; caspase; oxidative stress; inflammation; apoptosis; ascorbic acid; collagen; nitic oxide
资金
- University of Ferrara
- Programma Operativo Nazionale (PON) Ricerca e Innovazione 2014-2020, Fondo Sociale Europeo, Azione I. 2 Attrazione e Mobilita Internazionale dei Ricercatori
This study establishes a cell model to investigate the effects of hyaluronic acid (HA) on rotator cuff tendinopathy (RCT). The results show that HA with low molecular weight can reduce cell apoptosis, reverse the activation of caspase 3 and 7, and counteract the oxidative stress (OS) induced by RCT. Additionally, HA with higher molecular weight improves the expression of collagen 1. These findings enhance our understanding of the mechanisms of HA in the treatment of RCT.
Rotator cuff tendinopathy (RCT) is the primary reason for shoulder surgery and its clinical management is still challenging. Hyaluronic acid (HA) has been shown to have anti-inflammatory effects in vitro and in vivo under RCT conditions, characterized by an exaggerated oxidative stress (OS). However, molecular mechanisms underlying HA-related effects are still partially disclosed. With these aims, a cell model of RCT was established by exposing primary human tenocytes to H2O2 for up to 72 h. Four different HAs by molecular weight were administered to measure nitric oxide (NO) and OS, apoptosis, and collagen 1 expression. In parallel, the well-known antioxidant ascorbic acid was administered for comparison. The present study highlights that HAs characterized by a low molecular weight are able to counteract the H2O2-induced OS by decreasing the percentage of apoptotic cells and reversing the activation of caspase 3 and 7. Likewise, NO intracellular levels are comparable to the ones of controls. In parallel, collagen 1 expression was ameliorated by HAs characterized by higher molecular weights compared to AA. These findings confirm that HA plays an antioxidant role comparable to AA depending on the molecular weight, and highlight the molecular mechanisms underlying the HA anti-apoptotic effects.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据